Inhibition of HIV-1 IIIb replication in AA-2 and NT-2 cells in culture by two ligands of poly (ADP-ribose) polymerase: 6-amino-1,2-benzopyrone and 5-iodo-6-amino-1,2-benzopyrone

Gerald A. Cole, Gerhard Bauer, Eva Kirsten, Jerome Mendeleyev, Pal I. Bauer, Kalman G. Buki, Alaeddin Hakam, Ernest Kun

Research output: Contribution to journalArticle

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Abstract

The effects of two adenosine diphosphoribose transferase (ADPRT) enzyme inhibitory ligands, 6-amino-1,2-benzopyrone and its 5-iodo-derivative, were determined in AA-2 and MT-2 cell cultures on the replication of HIV-1 IIIb, assayed by an immunochemical test for the HIV protein p24, and syncytium formation, characteristic of HIV-infected cells. Intracellular concentrations of both drugs were sufficient to inhibit poly(ADP-ribose) polymerase activity within the intact cell. Both drugs inhibited HIV replication parallel to their inhibitory potency on ADPRT, but distinct differences were ascertained between the two cell lines. In AA-2 cells both p24 and syncytium formation were depressed simultaneously, whereas in MT-2 cells only syncytium formation was inhibited by the drugs, and the p24 production, which remained unchanged during viral growth, was unaffected. Both drugs only moderately depressed the growth rate of the AA-2 and MT-2 cells and there was no detectable cellular toxicity. Results suggest the feasibility of the development of a new line of ADPRT ligand anti-HIV drugs that fundamentally differ in their mode of action from currently used chemotherapeutics.

Original languageEnglish (US)
Pages (from-to)504-514
Number of pages11
JournalBiochemical and Biophysical Research Communications
Volume180
Issue number2
DOIs
StatePublished - Oct 31 1991

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Poly(ADP-ribose) Polymerases
Adenosine Diphosphate Ribose
HIV-1
Transferases
Cell Culture Techniques
Ligands
Giant Cells
Pharmaceutical Preparations
HIV Core Protein p24
Anti-HIV Agents
HIV
Cell culture
Toxicity
Growth
Cells
Derivatives
methyl(arginyl)-lysyl-prolyl-tryptophyl-tert-leucyl-leucine
5-iodo-6-amino-1,2-benzopyrone
6-amino-1,2-benzopyrone
Enzymes

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Inhibition of HIV-1 IIIb replication in AA-2 and NT-2 cells in culture by two ligands of poly (ADP-ribose) polymerase : 6-amino-1,2-benzopyrone and 5-iodo-6-amino-1,2-benzopyrone. / Cole, Gerald A.; Bauer, Gerhard; Kirsten, Eva; Mendeleyev, Jerome; Bauer, Pal I.; Buki, Kalman G.; Hakam, Alaeddin; Kun, Ernest.

In: Biochemical and Biophysical Research Communications, Vol. 180, No. 2, 31.10.1991, p. 504-514.

Research output: Contribution to journalArticle

Cole, Gerald A. ; Bauer, Gerhard ; Kirsten, Eva ; Mendeleyev, Jerome ; Bauer, Pal I. ; Buki, Kalman G. ; Hakam, Alaeddin ; Kun, Ernest. / Inhibition of HIV-1 IIIb replication in AA-2 and NT-2 cells in culture by two ligands of poly (ADP-ribose) polymerase : 6-amino-1,2-benzopyrone and 5-iodo-6-amino-1,2-benzopyrone. In: Biochemical and Biophysical Research Communications. 1991 ; Vol. 180, No. 2. pp. 504-514.
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