Inhibition of Gastric Motor Function by Circulating Corticotropin‐Releasing Factor in Anesthetized Rats

Helen E Raybould, C. B. Koelbel, E. A. Mayer, Y. Taché

Research output: Contribution to journalArticlepeer-review

1 Scopus citations


We have investigated the influence of intravenous corticotropin‐releasing factor (CRF) on intraluminal pressure of the body of the stomach and the neurohumoral pathways through which CRF inhibits gastric motor function in rats. CRF (0.21–210 pmol) injected intravenously produced a dose‐dependent, long‐lasting decrease in baseline tone and inhibited phasic gastric contractions measured manometrically in urethane‐anesthetized rats. The inhibitory effect of CRF was abolished by administration of hexamethonium or by subdiaphragmatic vagotomy but was unaffected by perineural treatment of the vagus nerves with capsaicin, adrenalectomy, or celiac/superior mesenteric ganglionectomy. Intravenous administration of CRF (126 pmol) inhibited by 69% the emptying of a noncaloric liquid meal as measured by the phenol red technique in conscious rats. Hexamethonium pretreatment significantly attenuated the CRF ‐induced delay in gastric emptying; bretylium, naloxone, adrenalectomy, and celiac I superior mesenteric ganglionectomy had no such effect. In an isolated tissue preparation, CRF (10−11 to 10−6 M) inhibited spontaneous, but not carbachol‐induced, contractions of rat antral longitudinal muscle. The effect of CRF was dose‐dependent and abolished in the presence of tetrodotoxin. These results suggest that peripheral CRF‐induced inhibition of corpus and antral motor contractility may mediate delayed gastric emptying via an action involving a nicotinic synapse, presumably in the enteric nervous system, that requires a vagal input. The in vitro studies support the concept that the action of CRF is not direct on smooth muscle but involves neural transmission within the enteric nervous system.

Original languageEnglish (US)
Pages (from-to)265-272
Number of pages8
JournalNeurogastroenterology and Motility
Issue number4
StatePublished - 1990


  • corticotropin‐releasing factor
  • gastric emptying
  • gastric smooth muscle
  • nicotinic
  • rat
  • vagus

ASJC Scopus subject areas

  • Physiology
  • Endocrine and Autonomic Systems
  • Gastroenterology


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