Inhibition of focal contact formation in cells transformed by p 185(neu)

Su Shun Lo, Su Hao Lo, Shao Chun Wang, Mien Chie Hung

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Signaling pathways mediated by adhesive molecules are tightly associated with cytoskeletal organization and cell growth regulation. Focal adhesion kinase (FAK) plays a prominent role in the adhesion signaling pathway through its tyrosine kinase activity and protein-protein interaction with other signaling molecules, including src, paxillin, and p130(CAS), and other proteins. We explored the roles of these signaling molecules in the transformation of B104-1-1 cells, an NIH/3T3-derived cell line transformed by activated rat p185(neu). The cytoskeletal organization of the p185(neu)- transformed cells was disrupted, and their morphology was dramatically altered. FAK, paxillin, and p130(CAS) appeared to be tyrosine phosphorylated in both NIH/3T3 and B104-1-1. However, the phosphorylation levels of paxillin and p130(CAS) were lower in B104-1-1 cells than in NIH/3T3 cells. Surprisingly, the association between FAK and paxillin was enhanced in B104- 1-1 cells, suggesting reorganization of protein-protein interaction modulated by protein phosphorylation. Our results showed that even though cellular transformation by src and neu has similar consequences, such as focal adhesion disassembly and increased metastasis potential, the molecular events underlying the signaling pathways can be dramatically different.

Original languageEnglish (US)
Pages (from-to)150-154
Number of pages5
JournalMolecular Carcinogenesis
Volume25
Issue number2
DOIs
StatePublished - 1999

Fingerprint

Focal Adhesions
Paxillin
Focal Adhesion Protein-Tyrosine Kinases
Proteins
Phosphorylation
3T3 Cells
NIH 3T3 Cells
Adhesives
Protein-Tyrosine Kinases
Tyrosine
Neoplasm Metastasis
Cell Line
Growth

Keywords

  • B104-1- 1
  • Focal adhesion kinase
  • P130(CAS)
  • P185(neu)
  • Paxillin
  • Phosphorylation
  • Src

ASJC Scopus subject areas

  • Cancer Research
  • Molecular Biology

Cite this

Inhibition of focal contact formation in cells transformed by p 185(neu). / Lo, Su Shun; Lo, Su Hao; Wang, Shao Chun; Hung, Mien Chie.

In: Molecular Carcinogenesis, Vol. 25, No. 2, 1999, p. 150-154.

Research output: Contribution to journalArticle

Lo, Su Shun ; Lo, Su Hao ; Wang, Shao Chun ; Hung, Mien Chie. / Inhibition of focal contact formation in cells transformed by p 185(neu). In: Molecular Carcinogenesis. 1999 ; Vol. 25, No. 2. pp. 150-154.
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