Inhibition of fibroblast proliferation in vitro using red light-emitting diodes

Hadar Lev-Tov, Andrew Mamalis, Neil Brody, Daniel Siegel, Jared Jagdeo

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Background Red light is part of the visible light spectrum. The effects of light-emitting diode (LED)-generated red light on human skin are not well-characterized. Objective To study the effect of red LED-generated low-level light therapy (LLLT) on fibroblast proliferation and viability in vitro. Methods and Materials Irradiation of normal human skin fibroblasts using red LED panels was performed in vitro, and modulation of proliferation and viability was quantified using trypan blue dye exclusion assay. Results Statistically significant decreases in cell proliferation were noted at the following fluences (time): 160 J/cm2 (30 minutes, 34 seconds), 320 J/cm2 (61 minutes, 07 seconds) and 640 J/cm2 (122 minutes, 14 seconds) (Figure 1). Irradiation at the 160- (98.5 ± 1.2%) and 320-J/cm2 (98.0 ± 3.1%) doses did not significantly alter viability. Conclusion At certain fluences, red LLLT can effectively inhibit fibroblast proliferation in vitro without altering viability and holds promise for the treatment of scars and other proliferative skin diseases.

Original languageEnglish (US)
Pages (from-to)1167-1170
Number of pages4
JournalDermatologic Surgery
Volume39
Issue number8
DOIs
StatePublished - Aug 2013

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Fibroblasts
Light
Skin
Trypan Blue
Skin Diseases
Cicatrix
Coloring Agents
Cell Proliferation
In Vitro Techniques
Low-Level Light Therapy
Therapeutics

ASJC Scopus subject areas

  • Dermatology
  • Surgery

Cite this

Inhibition of fibroblast proliferation in vitro using red light-emitting diodes. / Lev-Tov, Hadar; Mamalis, Andrew; Brody, Neil; Siegel, Daniel; Jagdeo, Jared.

In: Dermatologic Surgery, Vol. 39, No. 8, 08.2013, p. 1167-1170.

Research output: Contribution to journalArticle

Lev-Tov, Hadar ; Mamalis, Andrew ; Brody, Neil ; Siegel, Daniel ; Jagdeo, Jared. / Inhibition of fibroblast proliferation in vitro using red light-emitting diodes. In: Dermatologic Surgery. 2013 ; Vol. 39, No. 8. pp. 1167-1170.
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abstract = "Background Red light is part of the visible light spectrum. The effects of light-emitting diode (LED)-generated red light on human skin are not well-characterized. Objective To study the effect of red LED-generated low-level light therapy (LLLT) on fibroblast proliferation and viability in vitro. Methods and Materials Irradiation of normal human skin fibroblasts using red LED panels was performed in vitro, and modulation of proliferation and viability was quantified using trypan blue dye exclusion assay. Results Statistically significant decreases in cell proliferation were noted at the following fluences (time): 160 J/cm2 (30 minutes, 34 seconds), 320 J/cm2 (61 minutes, 07 seconds) and 640 J/cm2 (122 minutes, 14 seconds) (Figure 1). Irradiation at the 160- (98.5 ± 1.2{\%}) and 320-J/cm2 (98.0 ± 3.1{\%}) doses did not significantly alter viability. Conclusion At certain fluences, red LLLT can effectively inhibit fibroblast proliferation in vitro without altering viability and holds promise for the treatment of scars and other proliferative skin diseases.",
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