Inhibition of chemokine-induced chemotaxis of monkey leukocytes by μ-opioid receptor agonists

Yeon Choi, Linda F. Chuang, Kenneth M. Lam, Hsiang Fu Kung, Ji Ming Wang, Bennie Osburn, Ronald Y. Chuang

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

It is recognized that chemotaxis and phagocytosis constitute the first line of defense in the immune system, and chemokines function mainly as chemoattractants for phagocytic cells, recruiting monocytes and neutrophils from the blood to sites of infection. In this study, chemotaxis of monkey leukocytes was evaluated using human chemokines IL-8 (interleukin-8), MIP-1B and RANTES as the chemoattractants, and the effects of μ-opioid receptor agonists, morphine DAMGO, methadone and endomorphine, on the efficiency of chemotaxis were examined. It was found that human chemokines sewed well as chemoattractants for monkey leukocytes, and similar to the human system, chemokine-induced chemotaxis of monkey leukocytes was inhibited in the presence of μ-opioid receptor agonists. The inhibition could be reversed by naloxone, a specific μ-opioid receptor antagoinst. These studies further support the value of the monkey model for drug abuse studies in humans, as well as suggest that opioids such as morphine may alter immune functions through μ-opioid receptors on leukocytes.

Original languageEnglish (US)
Pages (from-to)389-396
Number of pages8
JournalIn Vivo
Volume13
Issue number5
StatePublished - Sep 1 1999

Fingerprint

Leukocyte Chemotaxis
Opioid Receptors
Chemokines
Haplorhini
Chemotactic Factors
Chemotaxis
Morphine
Leukocytes
Ala(2)-MePhe(4)-Gly(5)-enkephalin
Chemokine CCL5
Immune system
Methadone
Phagocytes
Naloxone
Interleukin-8
Phagocytosis
Opioid Analgesics
Substance-Related Disorders
Monocytes
Immune System

Keywords

  • Chemokines
  • Chemotaxis
  • Monkey leukocytes
  • Opioids

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology

Cite this

Choi, Y., Chuang, L. F., Lam, K. M., Kung, H. F., Wang, J. M., Osburn, B., & Chuang, R. Y. (1999). Inhibition of chemokine-induced chemotaxis of monkey leukocytes by μ-opioid receptor agonists. In Vivo, 13(5), 389-396.

Inhibition of chemokine-induced chemotaxis of monkey leukocytes by μ-opioid receptor agonists. / Choi, Yeon; Chuang, Linda F.; Lam, Kenneth M.; Kung, Hsiang Fu; Wang, Ji Ming; Osburn, Bennie; Chuang, Ronald Y.

In: In Vivo, Vol. 13, No. 5, 01.09.1999, p. 389-396.

Research output: Contribution to journalArticle

Choi, Y, Chuang, LF, Lam, KM, Kung, HF, Wang, JM, Osburn, B & Chuang, RY 1999, 'Inhibition of chemokine-induced chemotaxis of monkey leukocytes by μ-opioid receptor agonists', In Vivo, vol. 13, no. 5, pp. 389-396.
Choi Y, Chuang LF, Lam KM, Kung HF, Wang JM, Osburn B et al. Inhibition of chemokine-induced chemotaxis of monkey leukocytes by μ-opioid receptor agonists. In Vivo. 1999 Sep 1;13(5):389-396.
Choi, Yeon ; Chuang, Linda F. ; Lam, Kenneth M. ; Kung, Hsiang Fu ; Wang, Ji Ming ; Osburn, Bennie ; Chuang, Ronald Y. / Inhibition of chemokine-induced chemotaxis of monkey leukocytes by μ-opioid receptor agonists. In: In Vivo. 1999 ; Vol. 13, No. 5. pp. 389-396.
@article{68188fd7adcd49f5b0cd026435651c90,
title = "Inhibition of chemokine-induced chemotaxis of monkey leukocytes by μ-opioid receptor agonists",
abstract = "It is recognized that chemotaxis and phagocytosis constitute the first line of defense in the immune system, and chemokines function mainly as chemoattractants for phagocytic cells, recruiting monocytes and neutrophils from the blood to sites of infection. In this study, chemotaxis of monkey leukocytes was evaluated using human chemokines IL-8 (interleukin-8), MIP-1B and RANTES as the chemoattractants, and the effects of μ-opioid receptor agonists, morphine DAMGO, methadone and endomorphine, on the efficiency of chemotaxis were examined. It was found that human chemokines sewed well as chemoattractants for monkey leukocytes, and similar to the human system, chemokine-induced chemotaxis of monkey leukocytes was inhibited in the presence of μ-opioid receptor agonists. The inhibition could be reversed by naloxone, a specific μ-opioid receptor antagoinst. These studies further support the value of the monkey model for drug abuse studies in humans, as well as suggest that opioids such as morphine may alter immune functions through μ-opioid receptors on leukocytes.",
keywords = "Chemokines, Chemotaxis, Monkey leukocytes, Opioids",
author = "Yeon Choi and Chuang, {Linda F.} and Lam, {Kenneth M.} and Kung, {Hsiang Fu} and Wang, {Ji Ming} and Bennie Osburn and Chuang, {Ronald Y.}",
year = "1999",
month = "9",
day = "1",
language = "English (US)",
volume = "13",
pages = "389--396",
journal = "In Vivo",
issn = "0258-851X",
publisher = "International Institute of Anticancer Research",
number = "5",

}

TY - JOUR

T1 - Inhibition of chemokine-induced chemotaxis of monkey leukocytes by μ-opioid receptor agonists

AU - Choi, Yeon

AU - Chuang, Linda F.

AU - Lam, Kenneth M.

AU - Kung, Hsiang Fu

AU - Wang, Ji Ming

AU - Osburn, Bennie

AU - Chuang, Ronald Y.

PY - 1999/9/1

Y1 - 1999/9/1

N2 - It is recognized that chemotaxis and phagocytosis constitute the first line of defense in the immune system, and chemokines function mainly as chemoattractants for phagocytic cells, recruiting monocytes and neutrophils from the blood to sites of infection. In this study, chemotaxis of monkey leukocytes was evaluated using human chemokines IL-8 (interleukin-8), MIP-1B and RANTES as the chemoattractants, and the effects of μ-opioid receptor agonists, morphine DAMGO, methadone and endomorphine, on the efficiency of chemotaxis were examined. It was found that human chemokines sewed well as chemoattractants for monkey leukocytes, and similar to the human system, chemokine-induced chemotaxis of monkey leukocytes was inhibited in the presence of μ-opioid receptor agonists. The inhibition could be reversed by naloxone, a specific μ-opioid receptor antagoinst. These studies further support the value of the monkey model for drug abuse studies in humans, as well as suggest that opioids such as morphine may alter immune functions through μ-opioid receptors on leukocytes.

AB - It is recognized that chemotaxis and phagocytosis constitute the first line of defense in the immune system, and chemokines function mainly as chemoattractants for phagocytic cells, recruiting monocytes and neutrophils from the blood to sites of infection. In this study, chemotaxis of monkey leukocytes was evaluated using human chemokines IL-8 (interleukin-8), MIP-1B and RANTES as the chemoattractants, and the effects of μ-opioid receptor agonists, morphine DAMGO, methadone and endomorphine, on the efficiency of chemotaxis were examined. It was found that human chemokines sewed well as chemoattractants for monkey leukocytes, and similar to the human system, chemokine-induced chemotaxis of monkey leukocytes was inhibited in the presence of μ-opioid receptor agonists. The inhibition could be reversed by naloxone, a specific μ-opioid receptor antagoinst. These studies further support the value of the monkey model for drug abuse studies in humans, as well as suggest that opioids such as morphine may alter immune functions through μ-opioid receptors on leukocytes.

KW - Chemokines

KW - Chemotaxis

KW - Monkey leukocytes

KW - Opioids

UR - http://www.scopus.com/inward/record.url?scp=0033381795&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033381795&partnerID=8YFLogxK

M3 - Article

VL - 13

SP - 389

EP - 396

JO - In Vivo

JF - In Vivo

SN - 0258-851X

IS - 5

ER -