Inhibition of chemokine-induced chemotaxis of monkey leukocytes by μ-opioid receptor agonists

Yeon Choi, Linda F. Chuang, Kenneth M. Lam, Hsiang Fu Kung, Ji Ming Wang, Bennie Osburn, Ronald Y. Chuang

Research output: Contribution to journalArticle

38 Scopus citations

Abstract

It is recognized that chemotaxis and phagocytosis constitute the first line of defense in the immune system, and chemokines function mainly as chemoattractants for phagocytic cells, recruiting monocytes and neutrophils from the blood to sites of infection. In this study, chemotaxis of monkey leukocytes was evaluated using human chemokines IL-8 (interleukin-8), MIP-1B and RANTES as the chemoattractants, and the effects of μ-opioid receptor agonists, morphine DAMGO, methadone and endomorphine, on the efficiency of chemotaxis were examined. It was found that human chemokines sewed well as chemoattractants for monkey leukocytes, and similar to the human system, chemokine-induced chemotaxis of monkey leukocytes was inhibited in the presence of μ-opioid receptor agonists. The inhibition could be reversed by naloxone, a specific μ-opioid receptor antagoinst. These studies further support the value of the monkey model for drug abuse studies in humans, as well as suggest that opioids such as morphine may alter immune functions through μ-opioid receptors on leukocytes.

Original languageEnglish (US)
Pages (from-to)389-396
Number of pages8
JournalIn Vivo
Volume13
Issue number5
StatePublished - Sep 1 1999

Keywords

  • Chemokines
  • Chemotaxis
  • Monkey leukocytes
  • Opioids

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology

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    Choi, Y., Chuang, L. F., Lam, K. M., Kung, H. F., Wang, J. M., Osburn, B., & Chuang, R. Y. (1999). Inhibition of chemokine-induced chemotaxis of monkey leukocytes by μ-opioid receptor agonists. In Vivo, 13(5), 389-396.