Inhibition of aquaporin-4 significantly increases regional cerebral blood flow

Hironaka Igarashi, Mika Tsujita, Yuji Suzuki, Ingrid Kwee, Tsutomu Nakada

Research output: Contribution to journalArticle

32 Scopus citations

Abstract

The effects of the aquaporin-4 (AQP-4) inhibitor TGN-020 on regional cerebral blood flow (rCBF) was examined in wild-type (WT) and AQP-4 knockout (KO) mice in vivo. Although baseline absolute rCBF of WT and KO mice were equivalent (158.9±17.7 and 155.5±10.4 ml/100 g/min, respectively), TGN-020 produced a significant increase in rCBF compared with saline-treated WT mice (control), reaching a plateau 20 min after administration (118.45±8.13%, P<0.01). TGN-020 showed no effect on KO mice, supporting the concept that the observed increase in rCBF in WT mice was AQP-4 dependent. Administration of acetazolamide (positive control) produced an even greater increase in rCBF in WT compared with TGN-020 and a similar response in KO mice as well, reaching a sustained plateau 5 min after administration (138.50±9.75 and 138.52±9.76%, respectively, P<0.01 compared with baseline or saline-treated control mice). The study demonstrated that AQP-4 plays a role in regulation of rCBF.

Original languageEnglish (US)
Pages (from-to)324-328
Number of pages5
JournalNeuroReport
Volume24
Issue number6
DOIs
StatePublished - Apr 17 2013

Keywords

  • acetazolamide
  • aquaporin
  • regional cerebral blood flow
  • TGN-020

ASJC Scopus subject areas

  • Neuroscience(all)

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