Inhibition monitoring in veterinary molecular testing

Lifang Yan, Kathy L. Toohey-Kurth, Beate M. Crossley, Jianfa Bai, Amy L. Glaser, Rebecca L. Tallmadge, Laura B. Goodman

Research output: Contribution to journalArticle

Abstract

Many of the sample matrices typically used for veterinary molecular testing contain inhibitory factors that can potentially reduce analytic sensitivity or produce false-negative results by masking the signal produced by the nucleic acid target. Inclusion of internal controls in PCR-based assays is a valuable strategy not only for monitoring for PCR inhibitors, but also for monitoring nucleic acid extraction efficiency, and for identifying technology errors that may interfere with the ability of an assay to detect the intended target. The Laboratory Technology Committee of the American Association of Veterinary Laboratory Diagnosticians reviewed the different types of internal controls related to monitoring inhibition of PCR-based assays, and provides information here to encourage veterinary diagnostic laboratories to incorporate PCR internal control strategies as a routine quality management component of their molecular testing.

Original languageEnglish (US)
JournalJournal of Veterinary Diagnostic Investigation
DOIs
StateAccepted/In press - Jan 1 2019

Keywords

  • internal control
  • PCR inhibition
  • quality assurance
  • real-time PCR
  • veterinary molecular tests

ASJC Scopus subject areas

  • veterinary(all)

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