Inhibiting synthesis of extracellular matrix improves patch clamp seal formation

Ye Chen-Izu, Frederick Sachs

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

The ability to form gigaohm seals is essential for patch-clamp studies. Cells with otherwise useful properties must be abandoned for electrophysiological studies if seal formation is not possible. We have found that by inhibiting the growth of extracellular matrix with ß-D-xyloside, the success of forming gigaohm seals increased from near 0% to near 100%. Treated cells remained viable and appeared morphologically similar to untreated cells. Prototype treatment protocols are given for the renin secreting cell line As4.1.

Original languageEnglish (US)
Pages (from-to)218-220
Number of pages3
JournalPflügers Archiv European Journal of Physiology
Volume419
Issue number2
DOIs
StatePublished - Sep 1991
Externally publishedYes

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Keywords

  • channel
  • patch clamp
  • renin
  • seal

ASJC Scopus subject areas

  • Physiology

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