Abstract
Gap junctional intercellular communication (GJIC) may contribute to cellular differentiation. To examine this possibility in bone cells we examined markers of cellular differentiation, including alkaline phosphatase, osteocalcin, and osteopontin, in ROS17/2.8 cells (ROS), a rat osteoblastic cell line expressing phenotypic characteristics of fully differentiated osteoblasts. We utilized ROS rendered communication deficient either by stable transfection with antisense cDNA to connexin 43 (Cx43), the predominant gap junction protein in bone (RCx16 cells), or by overexpression of Cx45, a gap junction protein not normally expressed in ROS (ROS/Cx45 cells). Both RCx16 and ROS/Cx45 cells displayed reduced dye coupling and Cx43 protein expression relative to ROS, control transfectants, and ROS/Cx45tr, ROS cells expressing carboxylterminal truncated Cx45. Steady-state mRNA levels for osteocalcin as well as alkaline phosphatase activity, two markers of osteoblastic differentiation, were also reduced in poorly coupled RCx16 and ROS/Cx45 cells. On the other hand, steady-state mRNA levels for osteopontin increased slightly in RCx16 and ROS/Cx45 cells. These results suggest that GJIC at least partly contributes to the regulation of expression of markers of osteoblastic differentiation. Copyright (C) 1999 Elsevier Science Inc.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 661-666 |
| Number of pages | 6 |
| Journal | Bone |
| Volume | 25 |
| Issue number | 6 |
| DOIs | |
| State | Published - Dec 1999 |
| Externally published | Yes |
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Keywords
- Differentiation
- Gap junctions
- Osteoblasts
ASJC Scopus subject areas
- Physiology
- Hematology
Cite this
Inhibiting gap junctional intercellular communication alters expression of differentiation markers in osteoblastic cells. / Li, Z.; Zhou, Z.; Yellowley-genetos, Clare E; Donahue, H. J.
In: Bone, Vol. 25, No. 6, 12.1999, p. 661-666.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Inhibiting gap junctional intercellular communication alters expression of differentiation markers in osteoblastic cells
AU - Li, Z.
AU - Zhou, Z.
AU - Yellowley-genetos, Clare E
AU - Donahue, H. J.
PY - 1999/12
Y1 - 1999/12
N2 - Gap junctional intercellular communication (GJIC) may contribute to cellular differentiation. To examine this possibility in bone cells we examined markers of cellular differentiation, including alkaline phosphatase, osteocalcin, and osteopontin, in ROS17/2.8 cells (ROS), a rat osteoblastic cell line expressing phenotypic characteristics of fully differentiated osteoblasts. We utilized ROS rendered communication deficient either by stable transfection with antisense cDNA to connexin 43 (Cx43), the predominant gap junction protein in bone (RCx16 cells), or by overexpression of Cx45, a gap junction protein not normally expressed in ROS (ROS/Cx45 cells). Both RCx16 and ROS/Cx45 cells displayed reduced dye coupling and Cx43 protein expression relative to ROS, control transfectants, and ROS/Cx45tr, ROS cells expressing carboxylterminal truncated Cx45. Steady-state mRNA levels for osteocalcin as well as alkaline phosphatase activity, two markers of osteoblastic differentiation, were also reduced in poorly coupled RCx16 and ROS/Cx45 cells. On the other hand, steady-state mRNA levels for osteopontin increased slightly in RCx16 and ROS/Cx45 cells. These results suggest that GJIC at least partly contributes to the regulation of expression of markers of osteoblastic differentiation. Copyright (C) 1999 Elsevier Science Inc.
AB - Gap junctional intercellular communication (GJIC) may contribute to cellular differentiation. To examine this possibility in bone cells we examined markers of cellular differentiation, including alkaline phosphatase, osteocalcin, and osteopontin, in ROS17/2.8 cells (ROS), a rat osteoblastic cell line expressing phenotypic characteristics of fully differentiated osteoblasts. We utilized ROS rendered communication deficient either by stable transfection with antisense cDNA to connexin 43 (Cx43), the predominant gap junction protein in bone (RCx16 cells), or by overexpression of Cx45, a gap junction protein not normally expressed in ROS (ROS/Cx45 cells). Both RCx16 and ROS/Cx45 cells displayed reduced dye coupling and Cx43 protein expression relative to ROS, control transfectants, and ROS/Cx45tr, ROS cells expressing carboxylterminal truncated Cx45. Steady-state mRNA levels for osteocalcin as well as alkaline phosphatase activity, two markers of osteoblastic differentiation, were also reduced in poorly coupled RCx16 and ROS/Cx45 cells. On the other hand, steady-state mRNA levels for osteopontin increased slightly in RCx16 and ROS/Cx45 cells. These results suggest that GJIC at least partly contributes to the regulation of expression of markers of osteoblastic differentiation. Copyright (C) 1999 Elsevier Science Inc.
KW - Differentiation
KW - Gap junctions
KW - Osteoblasts
UR - http://www.scopus.com/inward/record.url?scp=0032725056&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032725056&partnerID=8YFLogxK
U2 - 10.1016/S8756-3282(99)00227-6
DO - 10.1016/S8756-3282(99)00227-6
M3 - Article
VL - 25
SP - 661
EP - 666
JO - Bone
JF - Bone
SN - 8756-3282
IS - 6
ER -