Ingested human insulin inhibits the mosquito NF-κB-dependent immune response to Plasmodium falciparum

Nazzy Pakpour, Vanessa Corby-Harris, Gabriel P. Green, Hannah M. Smithers, Kong W. Cheung, Michael A. Riehle, Shirley Luckhart

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

We showed previously that ingested human insulin activates the insulin/IGF-1 signaling pathway in Anopheles stephensi and increases the susceptibility of these mosquitoes to Plasmodium falciparum. In other organisms, insulin can alter immune responsiveness through regulation of NF-κB transcription factors, critical elements for innate immunity that are also central to mosquito immunity. We show here that insulin signaling decreased expression of NF-κB-regulated immune genes in mosquito cells stimulated with either bacterial or malarial soluble products. Further, human insulin suppressed mosquito immunity through sustained phosphatidylinositol 3-kinase activation, since inhibition of this pathway led to decreased parasite development in the mosquito. Together, these data demonstrate that activation of the insulin/IGF-1 signaling pathway by ingested human insulin can alter NF-κB-dependent immunity, and ultimately the susceptibility, of mosquitoes to P. falciparum.

Original languageEnglish (US)
Pages (from-to)2141-2149
Number of pages9
JournalInfection and Immunity
Volume80
Issue number6
DOIs
StatePublished - Jun 2012

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Plasmodium falciparum
Culicidae
Insulin
Immunity
Insulin-Like Growth Factor I
Phosphatidylinositol 3-Kinase
Anopheles
Innate Immunity
Parasites
Transcription Factors
Genes

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Parasitology
  • Infectious Diseases

Cite this

Pakpour, N., Corby-Harris, V., Green, G. P., Smithers, H. M., Cheung, K. W., Riehle, M. A., & Luckhart, S. (2012). Ingested human insulin inhibits the mosquito NF-κB-dependent immune response to Plasmodium falciparum. Infection and Immunity, 80(6), 2141-2149. https://doi.org/10.1128/IAI.06358-11

Ingested human insulin inhibits the mosquito NF-κB-dependent immune response to Plasmodium falciparum. / Pakpour, Nazzy; Corby-Harris, Vanessa; Green, Gabriel P.; Smithers, Hannah M.; Cheung, Kong W.; Riehle, Michael A.; Luckhart, Shirley.

In: Infection and Immunity, Vol. 80, No. 6, 06.2012, p. 2141-2149.

Research output: Contribution to journalArticle

Pakpour, N, Corby-Harris, V, Green, GP, Smithers, HM, Cheung, KW, Riehle, MA & Luckhart, S 2012, 'Ingested human insulin inhibits the mosquito NF-κB-dependent immune response to Plasmodium falciparum', Infection and Immunity, vol. 80, no. 6, pp. 2141-2149. https://doi.org/10.1128/IAI.06358-11
Pakpour N, Corby-Harris V, Green GP, Smithers HM, Cheung KW, Riehle MA et al. Ingested human insulin inhibits the mosquito NF-κB-dependent immune response to Plasmodium falciparum. Infection and Immunity. 2012 Jun;80(6):2141-2149. https://doi.org/10.1128/IAI.06358-11
Pakpour, Nazzy ; Corby-Harris, Vanessa ; Green, Gabriel P. ; Smithers, Hannah M. ; Cheung, Kong W. ; Riehle, Michael A. ; Luckhart, Shirley. / Ingested human insulin inhibits the mosquito NF-κB-dependent immune response to Plasmodium falciparum. In: Infection and Immunity. 2012 ; Vol. 80, No. 6. pp. 2141-2149.
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