Abstract
Oligosaccharides are known to play important roles in many biological processes. In the study of oligosaccharides, collision-induced dissociation (CID) is the most common dissociation method to elucidate the sequence and connectivity. However, a disadvantage of CID is the decrease in both the degree and efficiency of dissociation with increasing mass. In the present study, we have successfully performed infrared multiphoton dissociation (IRMPD) on 39 O-linked mucin-type oligosaccharide alditols (both neutral and anionic). CID and IRMPD spectra of several oligosaccharides were also compared. They yielded nearly identical fragment ions corresponding to the lowest energy fragmentation pathways. The characteristic fragmentations of structural motifs, which can provide the linkage information, were similarly presented in both CID and IRMPD spectra. Multistage of CID (MS3 or MS4) is commonly needed to completely sequence the oligosaccharides, while IRMPD of the same compounds yielded the fragment ions corresponding to the loss of the first residue to the last residue during a single-stage tandem MS (MS2). Finally, it is shown that the fragmentation efficiency of IRMPD increases with the increasing size of oligosaccharides.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 208-214 |
| Number of pages | 7 |
| Journal | Analytical Chemistry |
| Volume | 77 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 1 2005 |
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- Analytical Chemistry
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Infrared multiphoton dissociation of O-linked mucin-type oligosaccharides. / Zhang, Jinhua; Schubothe, Katherine; Li, Bensheng; Russell, Scott; Lebrilla, Carlito B.
In: Analytical Chemistry, Vol. 77, No. 1, 01.01.2005, p. 208-214.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Infrared multiphoton dissociation of O-linked mucin-type oligosaccharides
AU - Zhang, Jinhua
AU - Schubothe, Katherine
AU - Li, Bensheng
AU - Russell, Scott
AU - Lebrilla, Carlito B
PY - 2005/1/1
Y1 - 2005/1/1
N2 - Oligosaccharides are known to play important roles in many biological processes. In the study of oligosaccharides, collision-induced dissociation (CID) is the most common dissociation method to elucidate the sequence and connectivity. However, a disadvantage of CID is the decrease in both the degree and efficiency of dissociation with increasing mass. In the present study, we have successfully performed infrared multiphoton dissociation (IRMPD) on 39 O-linked mucin-type oligosaccharide alditols (both neutral and anionic). CID and IRMPD spectra of several oligosaccharides were also compared. They yielded nearly identical fragment ions corresponding to the lowest energy fragmentation pathways. The characteristic fragmentations of structural motifs, which can provide the linkage information, were similarly presented in both CID and IRMPD spectra. Multistage of CID (MS3 or MS4) is commonly needed to completely sequence the oligosaccharides, while IRMPD of the same compounds yielded the fragment ions corresponding to the loss of the first residue to the last residue during a single-stage tandem MS (MS2). Finally, it is shown that the fragmentation efficiency of IRMPD increases with the increasing size of oligosaccharides.
AB - Oligosaccharides are known to play important roles in many biological processes. In the study of oligosaccharides, collision-induced dissociation (CID) is the most common dissociation method to elucidate the sequence and connectivity. However, a disadvantage of CID is the decrease in both the degree and efficiency of dissociation with increasing mass. In the present study, we have successfully performed infrared multiphoton dissociation (IRMPD) on 39 O-linked mucin-type oligosaccharide alditols (both neutral and anionic). CID and IRMPD spectra of several oligosaccharides were also compared. They yielded nearly identical fragment ions corresponding to the lowest energy fragmentation pathways. The characteristic fragmentations of structural motifs, which can provide the linkage information, were similarly presented in both CID and IRMPD spectra. Multistage of CID (MS3 or MS4) is commonly needed to completely sequence the oligosaccharides, while IRMPD of the same compounds yielded the fragment ions corresponding to the loss of the first residue to the last residue during a single-stage tandem MS (MS2). Finally, it is shown that the fragmentation efficiency of IRMPD increases with the increasing size of oligosaccharides.
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UR - http://www.scopus.com/inward/citedby.url?scp=11844253194&partnerID=8YFLogxK
U2 - 10.1021/ac0489824
DO - 10.1021/ac0489824
M3 - Article
C2 - 15623298
AN - SCOPUS:11844253194
VL - 77
SP - 208
EP - 214
JO - Analytical Chemistry
JF - Analytical Chemistry
SN - 0003-2700
IS - 1
ER -