Infrared multiphoton dissociation mass spectrometry for structural elucidation of oligosaccharides

Bensheng Li, Hyun Joo An, Jerry L. Hedrick, Carlito B Lebrilla

Research output: Chapter in Book/Report/Conference proceedingChapter

13 Scopus citations


The structural elucidation of oligosaccharides remains a major challenge. Mass spectrometry provides a rapid and convenient method for structural elucidation on the basis of tandem mass spectrometry. Ions are commonly selected and subjected to collision-induced dissociation (CID) to obtain structural information. However, a disadvantage of CID is the decrease in both the degree and efficiency of dissociation with increasing mass. In this chapter, we illustrate the use of infrared multiphoton dissociation (IRMPD) to obtain structural information for O-and N-linked oligosaccharides. The IRMPD and CID behaviors of oligosaccharides are compared.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
Number of pages13
StatePublished - 2009

Publication series

NameMethods in Molecular Biology
ISSN (Print)10643745


  • Collision-induced dissociation
  • Cross-ring cleavage
  • Infrared multiphoton dissociation
  • Oligosaccharide

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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