Infrared multiphoton dissociation mass spectrometry for structural elucidation of oligosaccharides

Bensheng Li, Hyun Joo An, Jerry L. Hedrick, Carlito B Lebrilla

Research output: Chapter in Book/Report/Conference proceedingChapter

13 Scopus citations

Abstract

The structural elucidation of oligosaccharides remains a major challenge. Mass spectrometry provides a rapid and convenient method for structural elucidation on the basis of tandem mass spectrometry. Ions are commonly selected and subjected to collision-induced dissociation (CID) to obtain structural information. However, a disadvantage of CID is the decrease in both the degree and efficiency of dissociation with increasing mass. In this chapter, we illustrate the use of infrared multiphoton dissociation (IRMPD) to obtain structural information for O-and N-linked oligosaccharides. The IRMPD and CID behaviors of oligosaccharides are compared.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
Pages23-35
Number of pages13
Volume534
DOIs
StatePublished - 2009

Publication series

NameMethods in Molecular Biology
Volume534
ISSN (Print)10643745

Keywords

  • Collision-induced dissociation
  • Cross-ring cleavage
  • Infrared multiphoton dissociation
  • Oligosaccharide

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Fingerprint Dive into the research topics of 'Infrared multiphoton dissociation mass spectrometry for structural elucidation of oligosaccharides'. Together they form a unique fingerprint.

  • Cite this

    Li, B., An, H. J., Hedrick, J. L., & Lebrilla, C. B. (2009). Infrared multiphoton dissociation mass spectrometry for structural elucidation of oligosaccharides. In Methods in Molecular Biology (Vol. 534, pp. 23-35). (Methods in Molecular Biology; Vol. 534). https://doi.org/10.1007/978-1-59745-022-5_2