Influenza virus proteins

Identity, synthesis, and modification analyzed by two dimensional gel electrophoresis

M. L. Privalsky, E. E. Penhoet

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

A modification of the two-dimensional protein electrophoresis system of O'Farrell was used to resolve influenza A virus proteins from each other and from host proteins in infected cells. Viral protein spots corresponding to the hemagglutinin proteins, neuraminidase, nucleocapsid protein, and nonstructural protein, were identified on the two-dimensional electrophoretogram. Use of the two-dimensional separation has allowed the authors to identify glycoprotein heterogeneity, to demonstrate directly the synthesis of neuraminidase, to analyze some viral proteins early after infection, and to demonstrate that the influenza virus NP and NS proteins are phosphorylated in infected MDCK cells.

Original languageEnglish (US)
Pages (from-to)3625-3629
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume75
Issue number8
StatePublished - 1978

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Electrophoresis, Gel, Two-Dimensional
Orthomyxoviridae
Neuraminidase
Viral Proteins
Proteins
Nucleocapsid Proteins
Madin Darby Canine Kidney Cells
Influenza A virus
Hemagglutinins
Electrophoresis
Glycoproteins
Infection

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

Influenza virus proteins : Identity, synthesis, and modification analyzed by two dimensional gel electrophoresis. / Privalsky, M. L.; Penhoet, E. E.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 75, No. 8, 1978, p. 3625-3629.

Research output: Contribution to journalArticle

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