Influence of zinc on 17β estradiol and progesterone release from dispersed rat ovarian cells

D. DiPaolo, T. W. Graham, Frederick C Mohr, G. H. Stabenfeldt, Carl L Keen

Research output: Contribution to journalArticle

Abstract

To test the hypothesis that Zn can affect ovarian steroidogenesis, the influence of dietary Zn levels (+Zn: 25 μg Zn/g diet; -Zn: 0.5 μg Zn g/diet) and medium Zn concentrations on in vivo and in vitro 17β estradiol and progesterone release was examined. Maternal dietary treatment (0.5 μg Zn/g diet) was initiated on day 9 postnatal. Two days after injection with pregnant mare's serum gonadotropin, blood and ovaries were collected from immature rats (25-33 d old). Serum Zn, 17β estradiol, and progesterone were determined. Primary cultures of dispersed ovarian tissue were incubated in varying Zn concentrations (0.3, 5, 15, or 25 μmol/l) for 24 h, and media were collected for determination of 17β estradiol and progesterone. Serum Zn concentrations were lower in -Zn than in +Zn rats (5.1 ± 0.52 vs. 25.5 ± 1.29 μmol/l; P < .001). Serum concentrations of 17β estradiol (-Zn = 241 ± 25 pg/ml; +Zn = 249 ± 40 pg/ml; P > .05) and progesterone (-Zn = 8.49 ± 3.99 ng/ml; +Zn = 9.72 ± 3.22 ng/ml; P > .05) were similar between groups. In vitro 17β estradiol and progesterone release were not influenced by either dietary or media Zn treatment (P > .05).

Original languageEnglish (US)
Pages (from-to)147-154
Number of pages8
JournalJournal of Trace Elements in Experimental Medicine
Volume6
Issue number4
StatePublished - 1993

Keywords

  • Fertility
  • Folliculogenesis
  • Reproduction
  • Steroidogenesis

ASJC Scopus subject areas

  • Biochemistry

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