Influence of whole body irradiation and local shielding on matrix-induced endochondral bone differentiation

S. Wientroub, J. F. Weiss, G. N. Catravas, A Hari Reddi

Research output: Contribution to journalArticlepeer-review

22 Scopus citations


Subcutaneous implantation of demineralized bone matrix into allogeneic rats induces endochondral dochondral bone formation. We have investigated the effects of irradiation on the sequelae of the interaction of collagenous matrix and mesenchymal cells and on cartilage and bone differentiation. Rats were irradiated in a vertical direction with a midline dose of 850 rad. Radiation entered the rats ventrally while a small area of the upper thorax was locally shielded. After irradiation, bone matrix was implanted in shielded and nonshielded sites, and the implants were studied at various stages. On day 3, [3H]thymidine incorporation, an index of cell proliferation, was inhibited by 70% in the nonshielded sites compared to nonirradiated control rats. The degree of inhibition (35%) was less pronounced in shielded sites. Furthermore, there was recovery of cell proliferation in the shielded sites as opposed to the nonshielded contralateral site. A similar pattern was observed on day 7 as assessed by35SO4 incorporation into proteoglycans during chondrogenesis. Bone formation and mineralization were quantified on day 11 by alkaline phosphatase activity and45Ca incorporation. In nonshielded sites, there was a 73% inhibition of alkaline phosphatase activity. In conclusion, radiation impaired progenitor cell proliferation which resulted in decreased cartilage and bone differentiation. These findings imply that local mesenchymal cells proliferate and differentiate into bone in response to implanted collagenous matrix.

Original languageEnglish (US)
Pages (from-to)38-45
Number of pages8
JournalCalcified Tissue International
Issue number1
StatePublished - Jan 1990
Externally publishedYes


  • Combined injury
  • Matrix-induced endochondral bone formation
  • Radiation

ASJC Scopus subject areas

  • Endocrinology
  • Orthopedics and Sports Medicine
  • Endocrinology, Diabetes and Metabolism


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