Influence of vitamin A on matrix-induced endochondral bone formation

D. P. DeSimone, A Hari Reddi

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

The influence of retinoic acid on matrix-induced endochondral bone differentiation was determined. Retinoic acid was administered during discrete stages of endochondral bone formation, specifically, mesenchymal cell proliferation, chondrogenesis, bone formation, and mineralization. In retinoic acid-treated rats examined on day 3 following matrix implantation, biochemical markers for mesenchymal cell proliferation were about 50% of the controls. Chondrogenesis on day 7, assessed by35SO4 incorporation into proteoglycans, was 27% of the control. In addition, dissociative extraction of proteoglycans with 4.0 M guanidine-HCl and chromatography on Sepharose CL-2B revealed the synthesis of a smaller molecular weight proteoglycan when compared to controls which exhibited the cartilage-specific type. Osteogenesis and bone mineralization were monitored by alkaline phosphatase activity and45Ca incorporation. On day 11 alkaline phosphatase activity was decreased by 40% and45Ca incorporation was 48% of the control. These results revealed the multiple foci of the actions of excess vitamin A.

Original languageEnglish (US)
Pages (from-to)732-739
Number of pages8
JournalCalcified Tissue International
Volume35
Issue number1
DOIs
StatePublished - Dec 1983
Externally publishedYes

Keywords

  • Chondrogenesis
  • Mesenchymol cell proliferation
  • Osteogenesis
  • Proteoglycans

ASJC Scopus subject areas

  • Endocrinology
  • Orthopedics and Sports Medicine
  • Endocrinology, Diabetes and Metabolism

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