Influence of SLA haplotype on preimplantation embryonic cell number in miniature pigs

S. P. Ford, N. K. Schwartz, M. F. Rothschild, Alan J Conley, C. M. Warner

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Embryonic cello number in miniature pigs inbred for specific SLA haplotypes (a, c, and d) was determined on Day 6 by nuclear staining and, on Days 9 and 11, by DNA analyses (first day of oestrus = Day 0). Pigs exhibiting first behavioural oestrus at 08:00 h were hand-mated to an SLA homozygous boar 12 and 24 h later. Numbers of embryos flushed from uteri at 08:00-10:00 h on Days 6, 9 and 11 were greater (P < 0.05) for SLA(d) females than for SLAa or SLA(c) females, which did not differ (8.2 vs 6.8 and 6.2, respectively). Recovery rates (embryos recovered/CL number) were similar, averaging 75.8% for all three SLA haplotypes. Embryos from SLA(d) dams contained fewer blastomeres (23 cells) on Day 6 than did embryos from SLAa (89 cells) or SLA(c) (79 cells) females. The reduced cell numbers of SLA(d) vs SLAa or SLA(c) embryos continued to Day 9 (28 vs 107 and 67 ng DNA/embryo) and Day 11 (167 vs 674 and 586 ng DNA/embryo). These results suggest an effect of the SLA complex on preimplantation embryonic development.

Original languageEnglish (US)
Pages (from-to)99-104
Number of pages6
JournalJournal of Reproduction and Fertility
Volume84
Issue number1
StatePublished - 1988
Externally publishedYes

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Physiology
  • Embryology
  • Obstetrics and Gynecology

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