Influence of SLA haplotype on preimplantation embryonic cell number in miniature pigs

S. P. Ford, N. K. Schwartz, M. F. Rothschild, Alan J Conley, C. M. Warner

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Embryonic cello number in miniature pigs inbred for specific SLA haplotypes (a, c, and d) was determined on Day 6 by nuclear staining and, on Days 9 and 11, by DNA analyses (first day of oestrus = Day 0). Pigs exhibiting first behavioural oestrus at 08:00 h were hand-mated to an SLA homozygous boar 12 and 24 h later. Numbers of embryos flushed from uteri at 08:00-10:00 h on Days 6, 9 and 11 were greater (P < 0.05) for SLA(d) females than for SLAa or SLA(c) females, which did not differ (8.2 vs 6.8 and 6.2, respectively). Recovery rates (embryos recovered/CL number) were similar, averaging 75.8% for all three SLA haplotypes. Embryos from SLA(d) dams contained fewer blastomeres (23 cells) on Day 6 than did embryos from SLAa (89 cells) or SLA(c) (79 cells) females. The reduced cell numbers of SLA(d) vs SLAa or SLA(c) embryos continued to Day 9 (28 vs 107 and 67 ng DNA/embryo) and Day 11 (167 vs 674 and 586 ng DNA/embryo). These results suggest an effect of the SLA complex on preimplantation embryonic development.

Original languageEnglish (US)
Pages (from-to)99-104
Number of pages6
JournalJournal of Reproduction and Fertility
Volume84
Issue number1
StatePublished - 1988
Externally publishedYes

Fingerprint

Haplotypes
Swine
Embryonic Structures
Cell Count
Estrus
DNA
Blastomeres
Uterus
Embryonic Development
Hand
Staining and Labeling

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Physiology
  • Embryology
  • Obstetrics and Gynecology

Cite this

Ford, S. P., Schwartz, N. K., Rothschild, M. F., Conley, A. J., & Warner, C. M. (1988). Influence of SLA haplotype on preimplantation embryonic cell number in miniature pigs. Journal of Reproduction and Fertility, 84(1), 99-104.

Influence of SLA haplotype on preimplantation embryonic cell number in miniature pigs. / Ford, S. P.; Schwartz, N. K.; Rothschild, M. F.; Conley, Alan J; Warner, C. M.

In: Journal of Reproduction and Fertility, Vol. 84, No. 1, 1988, p. 99-104.

Research output: Contribution to journalArticle

Ford, SP, Schwartz, NK, Rothschild, MF, Conley, AJ & Warner, CM 1988, 'Influence of SLA haplotype on preimplantation embryonic cell number in miniature pigs', Journal of Reproduction and Fertility, vol. 84, no. 1, pp. 99-104.
Ford, S. P. ; Schwartz, N. K. ; Rothschild, M. F. ; Conley, Alan J ; Warner, C. M. / Influence of SLA haplotype on preimplantation embryonic cell number in miniature pigs. In: Journal of Reproduction and Fertility. 1988 ; Vol. 84, No. 1. pp. 99-104.
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