Influence of prior influenza vaccination on antibody and B-cell responses

Sanae Sasaki, Xiaosong He, Tyson H. Holmes, Cornelia L. Dekker, George W. Kemble, Ann M. Arvin, Harry B. Greenberg

Research output: Contribution to journalArticle

152 Citations (Scopus)

Abstract

Currently two vaccines, trivalent inactivated influenza vaccine (TIV) and live attenuated influenza vaccine (LAIV), are licensed in the USA. Despite previous studies on immune responses induced by these two vaccines, a comparative study of the influence of prior influenza vaccination on serum antibody and B-cell responses to new LAIV or TIV vaccination has not been reported. During the 2005/6 influenza season, we quantified the serum antibody and B-cell responses to LAIV or TIV in adults with differing influenza vaccination histories in the prior year: LAIV, TIV, or neither. Blood samples were collected on days 0, 7-9 and 21-35 after immunization and used for serum HAI assay and B-cell assays. Total and influenza-specific circulating IgG and IgA antibody secreting cells (ASC) in PBMC were detected by direct ELISPOT assay. Memory B-cells were also tested by ELISPOT after polyclonal stimulation of PBMC in vitro. Serum antibody, effector, and memory B-cell responses were greater in TIV recipients than LAIV recipients. Prior year TIV recipients had significantly higher baseline HAI titers, but lower HAI response after vaccination with either TIV or LAIV, and lower IgA ASC response after vaccination with TIV than prior year LAIV or no vaccination recipients. Lower levels of baseline HAI titer were associated with a greater fold-increase of HAI titer and ASC number after vaccination, which also differed by type of vaccine. Our findings suggest that the type of vaccine received in the prior year affects the serum antibody and the B-cell responses to subsequent vaccination. In particular, prior year TIV vaccination is associated with sustained higher HAI titer one year later but lower antibody response to new LAIV or TIV vaccination, and a lower effector B-cell response to new TIV but not LAIV vaccination.

Original languageEnglish (US)
Article numbere2975
JournalPLoS One
Volume3
Issue number8
DOIs
StatePublished - Aug 20 2008
Externally publishedYes

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Influenza Vaccines
influenza
Human Influenza
B-lymphocytes
Vaccination
B-Lymphocytes
vaccination
Cells
Inactivated Vaccines
vaccines
antibodies
Antibodies
Attenuated Vaccines
blood serum
Antibody-Producing Cells
Vaccines
B-Lymphocyte Subsets
Enzyme-Linked Immunospot Assay
Assays
Serum

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Sasaki, S., He, X., Holmes, T. H., Dekker, C. L., Kemble, G. W., Arvin, A. M., & Greenberg, H. B. (2008). Influence of prior influenza vaccination on antibody and B-cell responses. PLoS One, 3(8), [e2975]. https://doi.org/10.1371/journal.pone.0002975

Influence of prior influenza vaccination on antibody and B-cell responses. / Sasaki, Sanae; He, Xiaosong; Holmes, Tyson H.; Dekker, Cornelia L.; Kemble, George W.; Arvin, Ann M.; Greenberg, Harry B.

In: PLoS One, Vol. 3, No. 8, e2975, 20.08.2008.

Research output: Contribution to journalArticle

Sasaki, S, He, X, Holmes, TH, Dekker, CL, Kemble, GW, Arvin, AM & Greenberg, HB 2008, 'Influence of prior influenza vaccination on antibody and B-cell responses', PLoS One, vol. 3, no. 8, e2975. https://doi.org/10.1371/journal.pone.0002975
Sasaki S, He X, Holmes TH, Dekker CL, Kemble GW, Arvin AM et al. Influence of prior influenza vaccination on antibody and B-cell responses. PLoS One. 2008 Aug 20;3(8). e2975. https://doi.org/10.1371/journal.pone.0002975
Sasaki, Sanae ; He, Xiaosong ; Holmes, Tyson H. ; Dekker, Cornelia L. ; Kemble, George W. ; Arvin, Ann M. ; Greenberg, Harry B. / Influence of prior influenza vaccination on antibody and B-cell responses. In: PLoS One. 2008 ; Vol. 3, No. 8.
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