Influence of phosphocitrate, a potent inhibitor of hydroxyapatite crystal growth, on mineralization of cartilage and bone

A Hari Reddi, J. L. Meyer, W. P. Tew, J. E. Howard, A. L. Lehninger

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

An in vivo bone induction system was used to study the effect of phosphocitrate, a potent inhibitor of calcium phosphate crystallization, on the initial stages of mineralization. Subcutaneous transplantation of coarse powders of demineralized rat diaphyseal bone matrix into allogenic recipients results in a well defined sequence of mineralization steps involving cartilage formation, calcification and bone formation. Phosphocitrate when administered locally or systemically had no effect on the mineralization of either cartilage or bone. Dose levels were chosen to be consistent with that of a previous study which demonstrated that ethane-1-hydroxy-1, 1-diphosphonate(EHDP), a similarly effective in vitro inhibitor of hydroxyapatite crystallization, has a strong inhibitory effect on the initial mineralization steps. Since EHDP is not metabolized in vivo, the lack of effect of phosphocitrate in this study is suggestive of possible enzymatic hydrolysis at the calcification site which may regulate the level of phosphocitrate.

Original languageEnglish (US)
Pages (from-to)154-159
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume97
Issue number1
DOIs
StatePublished - 1980
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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