Influence of oligomer chain length on the antioxidant activity of procyanidins

Silvina B. Lotito, Lucas Actis-Goretta, M. Lourdes Renart, Marina Caligiuri, Dietrich Rein, Harold H. Schmitz, Francene M. Steinberg, Carl L Keen, Cesar G. Fraga

Research output: Contribution to journalArticlepeer-review

193 Scopus citations


The antioxidant activity of catechin monomers and procyanidin (dimers to hexamers) fractions purified from cocoa was studied in two in vitro systems: liposomes and human LDL. Liposome oxidation (evaluated as formation of 2-thiobarbituric acid reactive substances) was initiated with 2,2'-azobis (2-amidinopropane) hydrochloride (AAPH), 2,2'-azobis (2,4-dimethylvaleronitrile) (AMVN), iron/ascorbate, or UV-C; LDL oxidation (evaluated as formation of con jugated dienes) was initiated with Cu2+ or AAPH. Catechin monomers and procyanidin fractions inhibited both liposome and LDL oxidation. Monomers, dimers, and trimers fractions were the most effective antioxidants when llposome oxidation was initiated in the aqueous phase. When oxidation was initiated in the lipid domains, higher molecular weight procyanidins were the most effective. All fractions significantly inhibited Cu-mediated LDL oxidation; no significant effect of procyanidin molecular weight was observed. The hexamer fraction was the least effective with respect to preventing AAPH initiated LDL oxidation. Results reported herein give further evidence on the influence of the oligomer chain length on the antioxidant protection by procyanidins. (C) 2000 Academic Press.

Original languageEnglish (US)
Pages (from-to)945-951
Number of pages7
JournalBiochemical and Biophysical Research Communications
Issue number3
StatePublished - Oct 5 2000


  • Antioxidants
  • Catcchins
  • Cocoa
  • Procyanidins

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology


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