Influence of nonenzymatic glycation on biomechanical properties of cortical bone

D. Vashishth, G. J. Gibson, J. I. Khoury, M. B. Schaffler, J. Kimura, David P Fyhrie

Research output: Contribution to journalArticle

319 Citations (Scopus)

Abstract

In this study, the influence of nonenzymatic glycation (NEG) on the mechanical properties of bone and bone collagen were investigated. Bovine cortical bone specimens were incubated in ribose to cause collagen cross-links in vitro, and nondestructive mechanical testing was used to determine tensile and compressive elastic modulus as a function of incubation time. Mechanical properties associated with yield, postyield, and final fracture of bone were determined at the end of the incubation period. The stiffness of the collagen network was measured using stress relaxation tests of demineralized bone cylinders extracted periodically throughout the incubation period. It was found that accumulation of nonenzymatic glycation end-products in cortical bone caused stiffening of the type I collagen network in bone (r2 = 0.92; p < 0.001) but did not significantly affect the overall stiffness of the mineralized bone (p = 0.98). The ribosylated group had significantly more NEG products and higher yield stress and strain than the control group (p < 0.05). Postyield properties including postyield strain and strain energy were lower in the ribosylated group but were not significantly different from the control group (p = 0.24). Compared with the control group, the ribosylated group was characterized by significantly higher secant modulus and lower damage fraction (p < 0.05). Taken together, the results of this study suggest that collagen in bone is susceptible to the same NEG-mediated changes as collagen in other connective tissues and that an increased stiffness of the collagen network in bone due to NEG may explain some of the age-related increase in skeletal fragility and fracture risk.

Original languageEnglish (US)
Pages (from-to)195-201
Number of pages7
JournalBone
Volume28
Issue number2
DOIs
StatePublished - 2001
Externally publishedYes

Fingerprint

Collagen
Bone and Bones
Control Groups
Ribose
Elastic Modulus
Bone Fractures
Collagen Type I
Exercise Test
Connective Tissue
Cortical Bone

Keywords

  • Collagen
  • Cortical bone
  • Cross-link
  • Mechanical properties
  • Nonenzymatic glycation

ASJC Scopus subject areas

  • Physiology
  • Hematology

Cite this

Vashishth, D., Gibson, G. J., Khoury, J. I., Schaffler, M. B., Kimura, J., & Fyhrie, D. P. (2001). Influence of nonenzymatic glycation on biomechanical properties of cortical bone. Bone, 28(2), 195-201. https://doi.org/10.1016/S8756-3282(00)00434-8

Influence of nonenzymatic glycation on biomechanical properties of cortical bone. / Vashishth, D.; Gibson, G. J.; Khoury, J. I.; Schaffler, M. B.; Kimura, J.; Fyhrie, David P.

In: Bone, Vol. 28, No. 2, 2001, p. 195-201.

Research output: Contribution to journalArticle

Vashishth, D, Gibson, GJ, Khoury, JI, Schaffler, MB, Kimura, J & Fyhrie, DP 2001, 'Influence of nonenzymatic glycation on biomechanical properties of cortical bone', Bone, vol. 28, no. 2, pp. 195-201. https://doi.org/10.1016/S8756-3282(00)00434-8
Vashishth, D. ; Gibson, G. J. ; Khoury, J. I. ; Schaffler, M. B. ; Kimura, J. ; Fyhrie, David P. / Influence of nonenzymatic glycation on biomechanical properties of cortical bone. In: Bone. 2001 ; Vol. 28, No. 2. pp. 195-201.
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