Influence of nanomolar deltamethrin on the hallmarks of primary cultured cortical neuronal network and the role of ryanodine receptors

Jing Zheng, Yiyi Yu, Wei Feng, Jing Li, Ju Liu, Chunlei Zhang, Yao Dong, Isaac N Pessah, Zhengyu Cao

Research output: Contribution to journalArticle

Abstract

BACKGROUND: The pyrethroid deltamethrin (DM) is broadly used for insect control. Although DM hyperexcites neuronal networks by delaying inacti-vation of axonal voltage-dependent Na + channels, this mechanism is unlikely to mediate neurotoxicity at lower exposure levels during critical perinatal periods in mammals. OBJECTIVES: We aimed to identify mechanisms by which acute and subchronic DM altered axonal and dendritic growth, patterns of synchronous Ca2 + oscillations (SCOs), and electrical spike activity (ESA) functions critical to neuronal network formation. METHODS: Measurements of SCOs using Ca2 + imaging, ESA using microelectrode array (MEA) technology, and dendritic complexity using Sholl analysis were performed in primary murine cortical neurons from wild-type (WT) and/or ryanodine receptor 1 (RyR1T4826I/T4826I) mice between 5 and 14 d in vitro (DIV). ½3 HŠryanodine binding analysis and a single-channel voltage clamp were utilized to measure engagement of RyRs as a direct target of DM. RESULTS: Neuronal networks responded to DM (30–70 nM) as early as 5 DIV, reducing SCO amplitude and depressing ESA and burst frequencies by 60–70%. DM (10–300 nM) enhanced axonal growth in a nonmonotonic manner. DM ≥100 nM enhanced dendritic complexity. DM stabilized channel open states of RyR1, RyR2, and cortical preparations expressing all three isoforms. DM (30 nM) altered gating kinetics of RyR1 channels, increasing mean open time, decreasing mean closed time, and thereby enhancing overall open probability. SCO patterns from cortical networks expressing RyR1T4826I/T4826I were more responsive to DM than WT. RyR1T4826I/T4826I neurons showed inherently longer axonal lengths than WT neurons and maintained less length-promoting responses to nanomolar DM. CONCLUSIONS: Our findings suggested that RyRs were sensitive molecular targets of DM with functional consequences likely relevant for mediating abnormal neuronal network connectivity in vitro. https://doi.org/10.1289/EHP4583.

Original languageEnglish (US)
Article number067003
JournalEnvironmental health perspectives
Volume127
Issue number6
DOIs
StatePublished - Jun 1 2019

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Ryanodine Receptor Calcium Release Channel
Neurons
decamethrin
Insect Control
Pyrethrins
Microelectrodes
Growth
Mammals
Protein Isoforms

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Health, Toxicology and Mutagenesis

Cite this

Influence of nanomolar deltamethrin on the hallmarks of primary cultured cortical neuronal network and the role of ryanodine receptors. / Zheng, Jing; Yu, Yiyi; Feng, Wei; Li, Jing; Liu, Ju; Zhang, Chunlei; Dong, Yao; Pessah, Isaac N; Cao, Zhengyu.

In: Environmental health perspectives, Vol. 127, No. 6, 067003, 01.06.2019.

Research output: Contribution to journalArticle

Zheng, Jing ; Yu, Yiyi ; Feng, Wei ; Li, Jing ; Liu, Ju ; Zhang, Chunlei ; Dong, Yao ; Pessah, Isaac N ; Cao, Zhengyu. / Influence of nanomolar deltamethrin on the hallmarks of primary cultured cortical neuronal network and the role of ryanodine receptors. In: Environmental health perspectives. 2019 ; Vol. 127, No. 6.
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abstract = "BACKGROUND: The pyrethroid deltamethrin (DM) is broadly used for insect control. Although DM hyperexcites neuronal networks by delaying inacti-vation of axonal voltage-dependent Na + channels, this mechanism is unlikely to mediate neurotoxicity at lower exposure levels during critical perinatal periods in mammals. OBJECTIVES: We aimed to identify mechanisms by which acute and subchronic DM altered axonal and dendritic growth, patterns of synchronous Ca2 + oscillations (SCOs), and electrical spike activity (ESA) functions critical to neuronal network formation. METHODS: Measurements of SCOs using Ca2 + imaging, ESA using microelectrode array (MEA) technology, and dendritic complexity using Sholl analysis were performed in primary murine cortical neurons from wild-type (WT) and/or ryanodine receptor 1 (RyR1T4826I/T4826I) mice between 5 and 14 d in vitro (DIV). ½3 HŠryanodine binding analysis and a single-channel voltage clamp were utilized to measure engagement of RyRs as a direct target of DM. RESULTS: Neuronal networks responded to DM (30–70 nM) as early as 5 DIV, reducing SCO amplitude and depressing ESA and burst frequencies by 60–70{\%}. DM (10–300 nM) enhanced axonal growth in a nonmonotonic manner. DM ≥100 nM enhanced dendritic complexity. DM stabilized channel open states of RyR1, RyR2, and cortical preparations expressing all three isoforms. DM (30 nM) altered gating kinetics of RyR1 channels, increasing mean open time, decreasing mean closed time, and thereby enhancing overall open probability. SCO patterns from cortical networks expressing RyR1T4826I/T4826I were more responsive to DM than WT. RyR1T4826I/T4826I neurons showed inherently longer axonal lengths than WT neurons and maintained less length-promoting responses to nanomolar DM. CONCLUSIONS: Our findings suggested that RyRs were sensitive molecular targets of DM with functional consequences likely relevant for mediating abnormal neuronal network connectivity in vitro. https://doi.org/10.1289/EHP4583.",
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T1 - Influence of nanomolar deltamethrin on the hallmarks of primary cultured cortical neuronal network and the role of ryanodine receptors

AU - Zheng, Jing

AU - Yu, Yiyi

AU - Feng, Wei

AU - Li, Jing

AU - Liu, Ju

AU - Zhang, Chunlei

AU - Dong, Yao

AU - Pessah, Isaac N

AU - Cao, Zhengyu

PY - 2019/6/1

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N2 - BACKGROUND: The pyrethroid deltamethrin (DM) is broadly used for insect control. Although DM hyperexcites neuronal networks by delaying inacti-vation of axonal voltage-dependent Na + channels, this mechanism is unlikely to mediate neurotoxicity at lower exposure levels during critical perinatal periods in mammals. OBJECTIVES: We aimed to identify mechanisms by which acute and subchronic DM altered axonal and dendritic growth, patterns of synchronous Ca2 + oscillations (SCOs), and electrical spike activity (ESA) functions critical to neuronal network formation. METHODS: Measurements of SCOs using Ca2 + imaging, ESA using microelectrode array (MEA) technology, and dendritic complexity using Sholl analysis were performed in primary murine cortical neurons from wild-type (WT) and/or ryanodine receptor 1 (RyR1T4826I/T4826I) mice between 5 and 14 d in vitro (DIV). ½3 HŠryanodine binding analysis and a single-channel voltage clamp were utilized to measure engagement of RyRs as a direct target of DM. RESULTS: Neuronal networks responded to DM (30–70 nM) as early as 5 DIV, reducing SCO amplitude and depressing ESA and burst frequencies by 60–70%. DM (10–300 nM) enhanced axonal growth in a nonmonotonic manner. DM ≥100 nM enhanced dendritic complexity. DM stabilized channel open states of RyR1, RyR2, and cortical preparations expressing all three isoforms. DM (30 nM) altered gating kinetics of RyR1 channels, increasing mean open time, decreasing mean closed time, and thereby enhancing overall open probability. SCO patterns from cortical networks expressing RyR1T4826I/T4826I were more responsive to DM than WT. RyR1T4826I/T4826I neurons showed inherently longer axonal lengths than WT neurons and maintained less length-promoting responses to nanomolar DM. CONCLUSIONS: Our findings suggested that RyRs were sensitive molecular targets of DM with functional consequences likely relevant for mediating abnormal neuronal network connectivity in vitro. https://doi.org/10.1289/EHP4583.

AB - BACKGROUND: The pyrethroid deltamethrin (DM) is broadly used for insect control. Although DM hyperexcites neuronal networks by delaying inacti-vation of axonal voltage-dependent Na + channels, this mechanism is unlikely to mediate neurotoxicity at lower exposure levels during critical perinatal periods in mammals. OBJECTIVES: We aimed to identify mechanisms by which acute and subchronic DM altered axonal and dendritic growth, patterns of synchronous Ca2 + oscillations (SCOs), and electrical spike activity (ESA) functions critical to neuronal network formation. METHODS: Measurements of SCOs using Ca2 + imaging, ESA using microelectrode array (MEA) technology, and dendritic complexity using Sholl analysis were performed in primary murine cortical neurons from wild-type (WT) and/or ryanodine receptor 1 (RyR1T4826I/T4826I) mice between 5 and 14 d in vitro (DIV). ½3 HŠryanodine binding analysis and a single-channel voltage clamp were utilized to measure engagement of RyRs as a direct target of DM. RESULTS: Neuronal networks responded to DM (30–70 nM) as early as 5 DIV, reducing SCO amplitude and depressing ESA and burst frequencies by 60–70%. DM (10–300 nM) enhanced axonal growth in a nonmonotonic manner. DM ≥100 nM enhanced dendritic complexity. DM stabilized channel open states of RyR1, RyR2, and cortical preparations expressing all three isoforms. DM (30 nM) altered gating kinetics of RyR1 channels, increasing mean open time, decreasing mean closed time, and thereby enhancing overall open probability. SCO patterns from cortical networks expressing RyR1T4826I/T4826I were more responsive to DM than WT. RyR1T4826I/T4826I neurons showed inherently longer axonal lengths than WT neurons and maintained less length-promoting responses to nanomolar DM. CONCLUSIONS: Our findings suggested that RyRs were sensitive molecular targets of DM with functional consequences likely relevant for mediating abnormal neuronal network connectivity in vitro. https://doi.org/10.1289/EHP4583.

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