Influence of mouse apolipoprotein A-II on plasma lipoproteine in transgenic mice

Catherine C. Hedrick, Lawrence W. Castellani, Craig H Warden, Donald L. Puppione, Aldons J. Lusis

Research output: Contribution to journalArticle

141 Scopus citations

Abstract

The role of apolipoprotein A-II (apoA-II) in the metabolism of high density lipoproteins (HDL) is poorly understood. Previous studies of naturally occurring variations of apoA-II in mice have, however, suggested that apoA-II expression influences HDL size and concentration. We now provide definitive evidence of this using transgenic mice overexpressing mouse apoA-II. Thus, as compared with nontransgenic littermates, transgenic mice possessing 4-fold elevations of apoA-II mRNA and 2-3-fold elevations in plasma apoA-II levels exhibited more than a 2-fold increase in HDL levels as well as about a 25% increase in average HDL diameter. Overexpression of mouse apoA-II also resulted in a substantial decrease in the levels of apolipoprotein E associated with HDL, suggesting that apoA-II can displace apoE from HDL. The apoA-II transgenic mice also exhibited a 2-4-fold increase in plasma very low density and low density lipoprotein-cholesterol levels, suggesting novel interactions among the classes of plasma lipoproteins.

Original languageEnglish (US)
Pages (from-to)20676-20682
Number of pages7
JournalJournal of Biological Chemistry
Volume268
Issue number27
StatePublished - Sep 25 1993

ASJC Scopus subject areas

  • Biochemistry

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    Hedrick, C. C., Castellani, L. W., Warden, C. H., Puppione, D. L., & Lusis, A. J. (1993). Influence of mouse apolipoprotein A-II on plasma lipoproteine in transgenic mice. Journal of Biological Chemistry, 268(27), 20676-20682.