Influence of growth conditions on heat-stable phospholipase activity in Pseudomonas

R. Koka, Bart C Weimer

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Many psychrotrophic bacteria contaminating raw milk produce phospholipase that withstands pasteurization and UHT treatments. This enzyme acts on the milk fat globule membrane and exposes triacylglycerides to the action of lipase. Phospholipase production by various isolates of Pseudomonas was investigated. The isolates were cultured aerobically at 8 °C in nutrient broth, McKellar's minimal salts medium, Chrisope's medium, and skim milk. Each strain produced phospholipase during the 50 h incubation. Enzyme production varied significantly (P < 0·001) with strain and growth medium. Strains varied significantly (P < 0·001) in their enzyme production in each medium and during the incubation time as well. Strain, incubation time, and the growth medium significantly influenced (P < 0·001) heat stability of the enzyme activity. Pasteurization reduced the activity, but did not eliminate it in skim milk.

Original languageEnglish (US)
Pages (from-to)109-116
Number of pages8
JournalJournal of Dairy Research
Volume68
Issue number1
DOIs
StatePublished - 2001
Externally publishedYes

Fingerprint

Phospholipases
Pseudomonas
Pasteurization
Milk
Hot Temperature
heat
Enzymes
skim milk
pasteurization
Growth
Enzyme Stability
enzymes
culture media
UHT treatment
Lipase
psychrotrophic bacteria
heat stability
fat globules
Salts
raw milk

Keywords

  • Growth media
  • Lipolysis
  • Phospholipase
  • Pseudomonas
  • Raw milk

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Applied Microbiology and Biotechnology
  • Food Science

Cite this

Influence of growth conditions on heat-stable phospholipase activity in Pseudomonas. / Koka, R.; Weimer, Bart C.

In: Journal of Dairy Research, Vol. 68, No. 1, 2001, p. 109-116.

Research output: Contribution to journalArticle

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