Influence of extracellular matrix proteins and substratum topography on corneal epithelial cell alignment and migration

Vijaykrishna Raghunathan, Clayton McKee, Wai Cheung, Rachel Naik, Paul F. Nealey, Paul Russell, Christopher J Murphy

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The basement membrane (BM) of the corneal epithelium presents biophysical cues in the form of topography and compliance that can impact the phenotype and behaviors of cells and their nuclei through modulation of cytoskeletal dynamics. In addition, it is also well known that the intrinsic biochemical attributes of BMs can modulate cell behaviors. In this study, the influence of the combination of exogenous coating of extracellular matrix proteins (ECM) (fibronectin-collagen [FNC]) with substratum topography was investigated on cytoskeletal architecture as well as alignment and migration of immortalized corneal epithelial cells. In the absence of FNC coating, a significantly greater percentage of cells aligned parallel with the long axis of the underlying anisotropically ordered topographic features; however, their ability to migrate was impaired. Additionally, changes in the surface area, elongation, and orientation of cytoskeletal elements were differentially influenced by the presence or absence of FNC. These results suggest that the effects of topographic cues on cells are modulated by the presence of surface-associated ECM proteins. These findings have relevance to experiments using cell cultureware with biomimetic biophysical attributes as well as the integration of biophysical cues in tissue-engineering strategies and the development of improved prosthetics.

Original languageEnglish (US)
Pages (from-to)1713-1722
Number of pages10
JournalTissue Engineering - Part A
Volume19
Issue number15-16
DOIs
StatePublished - Aug 1 2013

Fingerprint

Corneal Topography
Extracellular Matrix Proteins
Fibronectins
Collagen
Topography
Cell Movement
Epithelial Cells
Proteins
Cues
Coatings
Biomimetics
Prosthetics
Tissue engineering
Corneal Epithelium
Aptitude
Elongation
Tissue Engineering
Cell Nucleus
Cells
Basement Membrane

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Biomedical Engineering
  • Biomaterials
  • Medicine(all)

Cite this

Influence of extracellular matrix proteins and substratum topography on corneal epithelial cell alignment and migration. / Raghunathan, Vijaykrishna; McKee, Clayton; Cheung, Wai; Naik, Rachel; Nealey, Paul F.; Russell, Paul; Murphy, Christopher J.

In: Tissue Engineering - Part A, Vol. 19, No. 15-16, 01.08.2013, p. 1713-1722.

Research output: Contribution to journalArticle

Raghunathan, Vijaykrishna ; McKee, Clayton ; Cheung, Wai ; Naik, Rachel ; Nealey, Paul F. ; Russell, Paul ; Murphy, Christopher J. / Influence of extracellular matrix proteins and substratum topography on corneal epithelial cell alignment and migration. In: Tissue Engineering - Part A. 2013 ; Vol. 19, No. 15-16. pp. 1713-1722.
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