Abstract
Objective-To determine biomechanical and biochemical properties of the medial meniscus in a semi-stable stifle model and in clinical patients and to determine the effect of canine recombinant somatotropin hormone (STH) on those properties. Animals-22 healthy adult dogs and 12 dogs with meniscal damage secondary to cranial cruciate ligament (CCL) rupture. Procedure-The CCL was transected in 15 dogs, and stifles were immediately stabilized. Implants releasing 4 mg of STH/d were placed in 7 dogs, and 8 received sham implants. Seven dogs were used as untreated controls. Force plate analysis was performed before surgery and 2, 5, and 10 weeks after surgery. After 10 weeks, dogs were euthanatized, and menisci from surgical and contralateral stifles were harvested. The torn caudal,horn of the medial meniscus in dogs with CCL rupture comprised the clinical group. Creep indentation determined aggregate modulus (HA), Poisson's ratio (v), permeability (k), and percentage recovery (%R). Water content (%W), collagen content (C), sulfated glycosaminoglycan (sGAG) content, and collagen type-I (cl) and -II (cll) immunoreactivity were also determined. Results-Surgical and clinical groups had lower HA, k, %R, C, sGAG, cl, and cll and higher %W than the nonsurgical group. Surgical stifles with greater weight bearing had stiffer menisci than those bearing less weight. Collagen content was higher in the surgical group receiving STH than the surgical group without STH. Conclusions and Clinical Relevance-Acute stabilization and moderate weight bearing of the CCL-deficient stifle appear to protect stiffness of the medial meniscus. Normal appearing menisci from CCL-deficient stifles can have alterations in biomechanical and biochemical properties, which may contribute to meniscal failure.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 419-426 |
| Number of pages | 8 |
| Journal | American Journal of Veterinary Research |
| Volume | 63 |
| Issue number | 3 |
| State | Published - Mar 1 2002 |
| Externally published | Yes |
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Influence of canine recombinant somatotropin hormone on biomechanical and biochemical properties of the medial meniscus in stifles with altered stability. / Noone, Thomas J.; Millis, Darryl L.; Korvick, Donna L.; Athanasiou, Kyriacos; Cook, James L.; Kuroki, Keiichi; Buonomo, Fran.
In: American Journal of Veterinary Research, Vol. 63, No. 3, 01.03.2002, p. 419-426.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Influence of canine recombinant somatotropin hormone on biomechanical and biochemical properties of the medial meniscus in stifles with altered stability
AU - Noone, Thomas J.
AU - Millis, Darryl L.
AU - Korvick, Donna L.
AU - Athanasiou, Kyriacos
AU - Cook, James L.
AU - Kuroki, Keiichi
AU - Buonomo, Fran
PY - 2002/3/1
Y1 - 2002/3/1
N2 - Objective-To determine biomechanical and biochemical properties of the medial meniscus in a semi-stable stifle model and in clinical patients and to determine the effect of canine recombinant somatotropin hormone (STH) on those properties. Animals-22 healthy adult dogs and 12 dogs with meniscal damage secondary to cranial cruciate ligament (CCL) rupture. Procedure-The CCL was transected in 15 dogs, and stifles were immediately stabilized. Implants releasing 4 mg of STH/d were placed in 7 dogs, and 8 received sham implants. Seven dogs were used as untreated controls. Force plate analysis was performed before surgery and 2, 5, and 10 weeks after surgery. After 10 weeks, dogs were euthanatized, and menisci from surgical and contralateral stifles were harvested. The torn caudal,horn of the medial meniscus in dogs with CCL rupture comprised the clinical group. Creep indentation determined aggregate modulus (HA), Poisson's ratio (v), permeability (k), and percentage recovery (%R). Water content (%W), collagen content (C), sulfated glycosaminoglycan (sGAG) content, and collagen type-I (cl) and -II (cll) immunoreactivity were also determined. Results-Surgical and clinical groups had lower HA, k, %R, C, sGAG, cl, and cll and higher %W than the nonsurgical group. Surgical stifles with greater weight bearing had stiffer menisci than those bearing less weight. Collagen content was higher in the surgical group receiving STH than the surgical group without STH. Conclusions and Clinical Relevance-Acute stabilization and moderate weight bearing of the CCL-deficient stifle appear to protect stiffness of the medial meniscus. Normal appearing menisci from CCL-deficient stifles can have alterations in biomechanical and biochemical properties, which may contribute to meniscal failure.
AB - Objective-To determine biomechanical and biochemical properties of the medial meniscus in a semi-stable stifle model and in clinical patients and to determine the effect of canine recombinant somatotropin hormone (STH) on those properties. Animals-22 healthy adult dogs and 12 dogs with meniscal damage secondary to cranial cruciate ligament (CCL) rupture. Procedure-The CCL was transected in 15 dogs, and stifles were immediately stabilized. Implants releasing 4 mg of STH/d were placed in 7 dogs, and 8 received sham implants. Seven dogs were used as untreated controls. Force plate analysis was performed before surgery and 2, 5, and 10 weeks after surgery. After 10 weeks, dogs were euthanatized, and menisci from surgical and contralateral stifles were harvested. The torn caudal,horn of the medial meniscus in dogs with CCL rupture comprised the clinical group. Creep indentation determined aggregate modulus (HA), Poisson's ratio (v), permeability (k), and percentage recovery (%R). Water content (%W), collagen content (C), sulfated glycosaminoglycan (sGAG) content, and collagen type-I (cl) and -II (cll) immunoreactivity were also determined. Results-Surgical and clinical groups had lower HA, k, %R, C, sGAG, cl, and cll and higher %W than the nonsurgical group. Surgical stifles with greater weight bearing had stiffer menisci than those bearing less weight. Collagen content was higher in the surgical group receiving STH than the surgical group without STH. Conclusions and Clinical Relevance-Acute stabilization and moderate weight bearing of the CCL-deficient stifle appear to protect stiffness of the medial meniscus. Normal appearing menisci from CCL-deficient stifles can have alterations in biomechanical and biochemical properties, which may contribute to meniscal failure.
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M3 - Article
C2 - 11911578
AN - SCOPUS:0036521188
VL - 63
SP - 419
EP - 426
JO - American Journal of Veterinary Research
JF - American Journal of Veterinary Research
SN - 0002-9645
IS - 3
ER -