Abstract
Dry ashing and wet ashing are two commonly used methods for the preparation of biological materials for trace element analysis by atomic absorption spectrophotometry. In this paper, National Bureau of Standards (NBS) bovine liver was dry ashed at 450°C for 24 h in silica glass (Vycor) or procelain crucibles; the resulting ash was dissolved in either concentrated nitric or hydrochloric acid. Dry ashing efficiency was evaluated by comparing iron, copper, zinc, and manganese concentrations of the samples with the values certified by NBS. Highest recoveries were obtained by dry ashing in silica glass (Vycor) crucibles. Dissolving the resultant ash in either hydrochloric or nitric acids did not significantly alter the results. A comparison between dry and wet ashing shows the latter method to be superior for the preparation of biological tissues for analysis of iron, copper, zinc, and manganese.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 237-244 |
| Number of pages | 8 |
| Journal | Biological Trace Element Research |
| Volume | 3 |
| Issue number | 3 |
| DOIs | |
| State | Published - Sep 1981 |
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Keywords
- analysis, of trace elements in biological samples
- Ashing techniques, and biotrace element analysis
- copper analysis, in biological samples
- dry ashing, in biotrace element analysis
- iron analysis, in biological samples
- manganese analysis, in biological samples
- trace element analysis, in biological samples
- zinc analysis, in biological samples
ASJC Scopus subject areas
- Endocrinology, Diabetes and Metabolism
- Biochemistry, medical
- Inorganic Chemistry
- Clinical Biochemistry
- Biochemistry
- Biochemistry, Genetics and Molecular Biology(all)
- Endocrinology
Cite this
Influence of ashing techniques on the analysis of trace elements in biological samples - II. Dry ashing. / Clegg, Michael S.; Keen, Carl L; Lönnerdal, Bo; Hurley, Lucille S.
In: Biological Trace Element Research, Vol. 3, No. 3, 09.1981, p. 237-244.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Influence of ashing techniques on the analysis of trace elements in biological samples - II. Dry ashing
AU - Clegg, Michael S.
AU - Keen, Carl L
AU - Lönnerdal, Bo
AU - Hurley, Lucille S.
PY - 1981/9
Y1 - 1981/9
N2 - Dry ashing and wet ashing are two commonly used methods for the preparation of biological materials for trace element analysis by atomic absorption spectrophotometry. In this paper, National Bureau of Standards (NBS) bovine liver was dry ashed at 450°C for 24 h in silica glass (Vycor) or procelain crucibles; the resulting ash was dissolved in either concentrated nitric or hydrochloric acid. Dry ashing efficiency was evaluated by comparing iron, copper, zinc, and manganese concentrations of the samples with the values certified by NBS. Highest recoveries were obtained by dry ashing in silica glass (Vycor) crucibles. Dissolving the resultant ash in either hydrochloric or nitric acids did not significantly alter the results. A comparison between dry and wet ashing shows the latter method to be superior for the preparation of biological tissues for analysis of iron, copper, zinc, and manganese.
AB - Dry ashing and wet ashing are two commonly used methods for the preparation of biological materials for trace element analysis by atomic absorption spectrophotometry. In this paper, National Bureau of Standards (NBS) bovine liver was dry ashed at 450°C for 24 h in silica glass (Vycor) or procelain crucibles; the resulting ash was dissolved in either concentrated nitric or hydrochloric acid. Dry ashing efficiency was evaluated by comparing iron, copper, zinc, and manganese concentrations of the samples with the values certified by NBS. Highest recoveries were obtained by dry ashing in silica glass (Vycor) crucibles. Dissolving the resultant ash in either hydrochloric or nitric acids did not significantly alter the results. A comparison between dry and wet ashing shows the latter method to be superior for the preparation of biological tissues for analysis of iron, copper, zinc, and manganese.
KW - analysis, of trace elements in biological samples
KW - Ashing techniques, and biotrace element analysis
KW - copper analysis, in biological samples
KW - dry ashing, in biotrace element analysis
KW - iron analysis, in biological samples
KW - manganese analysis, in biological samples
KW - trace element analysis, in biological samples
KW - zinc analysis, in biological samples
UR - http://www.scopus.com/inward/record.url?scp=0019769978&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0019769978&partnerID=8YFLogxK
U2 - 10.1007/BF02990120
DO - 10.1007/BF02990120
M3 - Article
AN - SCOPUS:0019769978
VL - 3
SP - 237
EP - 244
JO - Biological Trace Element Research
JF - Biological Trace Element Research
SN - 0163-4984
IS - 3
ER -