Influence of ashing techniques on the analysis of trace elements in animal tissue - I. Wet ashing

Michael S. Clegg, Carl L Keen, Bo Lönnerdal, Lucille S. Hurley

Research output: Contribution to journalArticlepeer-review

370 Scopus citations


A multitude of methods exists at present for the solubilization of biological tissues for atomic absorption analysis. We have examined several common methods of wet ashing using NBS bovine liver in order to determine which acids, acid combinations, or bases should be used as digesting agents for accurate and precise measurement of iron, copper, zinc, and manganese. Nitric acid proved to be the most effective wet ashing agent. With nitric acid, mean concentrations for iron, copper, and zinc differed from NBS certified values by less than 1.5% while those for manganese differed by 4%.

Original languageEnglish (US)
Pages (from-to)107-115
Number of pages9
JournalBiological Trace Element Research
Issue number2
StatePublished - Jun 1981


  • Analysis of trace elements, by wet ashing
  • copper, analysis by wet ashing
  • elemental analysis, trace, wet ashing in
  • iron, analysis by wet ashing
  • manganese, analysis by wet ashing
  • trace element analysis, by wet ashing
  • wet ashing techniques, in trace element analysis

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Endocrinology
  • Biochemistry, Genetics and Molecular Biology(all)


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