Influence of antioxidants on cadmium toxicity of mouse preimplantation embryos in vitro

Jeffrey M. Peters, John R. Duncan, Lynn M. Wiley, Carl L Keen

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

To test the hypothesis that the developmental toxicity of cadmium (Cd) is due in part to oxidative damage, embryos were cultured in medium containing 0.0, 1.0, 3.0, or 6.0 μM Cd with or without various antioxidants for 72 h. Ascorbate, butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT) and glutathione (GSH) were all effective at ameliorating 1.0 μM Cd-induced embryotoxicity. For embryos cultured in medium containing either 3.0 or 6.0 μM Cd, GSH was effective at ameliorating Cd toxicity while the other antioxidants tested were ineffective. Pretreating embryos with antioxidants for 24 h prior to exposing them to Cd and antioxidants did not significantly alter the previously observed improvement with the exception that pretreatment with GSH virtually eliminated Cd-induced embryotoxicity between 1.0 and 6.0 μM Cd. A 4-h exposure to GSH prior to culture in Cd markedly improved embryo development suggesting that GSH taken up during pretreatment can provide protection against Cd-induced embryotoxicity. This work supports the hypothesis that the developmental toxicity of Cd is in part due to oxidative damage that can be modulated by select antioxidants.

Original languageEnglish (US)
Pages (from-to)11-18
Number of pages8
JournalToxicology
Volume99
Issue number1-2
DOIs
StatePublished - May 5 1995

Fingerprint

Blastocyst
Cadmium
Toxicity
Antioxidants
Embryonic Structures
In Vitro Techniques
Butylated Hydroxyanisole
Butylated Hydroxytoluene
Embryonic Development
Glutathione

Keywords

  • Antioxidants
  • Cadmium
  • Development
  • Embryo
  • Glutathione
  • Oxidative damage

ASJC Scopus subject areas

  • Toxicology

Cite this

Influence of antioxidants on cadmium toxicity of mouse preimplantation embryos in vitro. / Peters, Jeffrey M.; Duncan, John R.; Wiley, Lynn M.; Keen, Carl L.

In: Toxicology, Vol. 99, No. 1-2, 05.05.1995, p. 11-18.

Research output: Contribution to journalArticle

Peters, Jeffrey M. ; Duncan, John R. ; Wiley, Lynn M. ; Keen, Carl L. / Influence of antioxidants on cadmium toxicity of mouse preimplantation embryos in vitro. In: Toxicology. 1995 ; Vol. 99, No. 1-2. pp. 11-18.
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