Influence of age and caloric restriction on liver glycolytic enzyme activities and metabolite concentrations in mice

Kevork Hagopian, Jon J Ramsey, Richard Weindruch

Research output: Contribution to journalArticlepeer-review

66 Scopus citations


The influence of caloric restriction (CR) from 2 months of age on the activities of liver glycolytic enzymes and metabolite levels was studied in young and old mice. Livers were sampled 48h after the last scheduled feeding time. Old mice on CR showed significant decreases in the activities of all the enzymes studied, except for aldolase, triosephosphate isomerase and phosphoglycerate mutase, which were unchanged. The metabolites glucose, glucose-6-phosphate, fructose-6-phosphate, pyruvate and lactate were lower while fructose-1,6-bisphosphate, glyceraldehyde-3-phosphate, dihydroxyacetone phosphate, 3-phosphoglycerate and phosphoenolpyruvate were increased in old CR. Young mice on CR also showed reduced enzyme activities, except for aldolase, triosephosphate isomerase and enolase which were unchanged when compared with young controls. The metabolites glucose, glucose-6-phosphate, fructose-6-phosphate and pyruvate were decreased when compared with young controls, while phosphoenolpyruvate was increased. Ketone bodies increased (65%) in old, but not young, CR mice while fructose-2,6-bisphosphate decreased in both young (22%) and old CR (28%) mice. The results indicate that decreased hepatic glucose levels in CR mice are associated with decreased enzyme activities but not a uniform decrease in metabolite levels. Increased ketone body levels indicate increased utilization of non-carbohydrate fuels while decreased fructose-2,6-bisphosphate level suggests its importance in the control of glycolysis in CR.

Original languageEnglish (US)
Pages (from-to)253-266
Number of pages14
JournalExperimental Gerontology
Issue number3
StatePublished - Mar 1 2003


  • Caloric restriction
  • Glucose
  • Glycolysis
  • Glycolytic enzymes
  • Glycolytic metabolites

ASJC Scopus subject areas

  • Aging
  • Medicine(all)


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