Inflammation-associated alterations to the intestinal microbiota reduce colonization resistance against non-typhoidal Salmonella during concurrent malaria parasite infection

Jason P. Mooney, Kristen L. Lokken, Mariana X. Byndloss, Michael D. George, Eric M. Velazquez, Franziska Faber, Brian P. Butler, Gregory T. Walker, Mohamed M. Ali, Rashaun Potts, Caitlin Tiffany, Brian M M Ahmer, Shirley Luckhart, Renee M Tsolis

Research output: Contribution to journalArticle

30 Scopus citations

Abstract

Childhood malaria is a risk factor for disseminated infections with non-typhoidal Salmonella (NTS) in sub-Saharan Africa. While hemolytic anemia and an altered cytokine environment have been implicated in increased susceptibility to NTS, it is not known whether malaria affects resistance to intestinal colonization with NTS. To address this question, we utilized a murine model of co-infection. Infection of mice with Plasmodium yoelii elicited infiltration of inflammatory macrophages and T cells into the intestinal mucosa and increased expression of inflammatory cytokines. These mucosal responses were also observed in germ-free mice, showing that they are independent of the resident microbiota. Remarkably, P. yoelii infection reduced colonization resistance of mice against S. enterica serotype Typhimurium. Further, 16S rRNA sequence analysis of the intestinal microbiota revealed marked changes in the community structure. Shifts in the microbiota increased susceptibility to intestinal colonization by S. Typhimurium, as demonstrated by microbiota reconstitution of germ-free mice. These results show that P. yoelii infection, via alterations to the microbial community in the intestine, decreases resistance to intestinal colonization with NTS. Further they raise the possibility that decreased colonization resistance may synergize with effects of malaria on systemic immunity to increase susceptibility to disseminated NTS infections.

Original languageEnglish (US)
Article number14603
JournalScientific Reports
Volume5
DOIs
StatePublished - Oct 5 2015

ASJC Scopus subject areas

  • General

Fingerprint Dive into the research topics of 'Inflammation-associated alterations to the intestinal microbiota reduce colonization resistance against non-typhoidal Salmonella during concurrent malaria parasite infection'. Together they form a unique fingerprint.

  • Cite this

    Mooney, J. P., Lokken, K. L., Byndloss, M. X., George, M. D., Velazquez, E. M., Faber, F., Butler, B. P., Walker, G. T., Ali, M. M., Potts, R., Tiffany, C., Ahmer, B. M. M., Luckhart, S., & Tsolis, R. M. (2015). Inflammation-associated alterations to the intestinal microbiota reduce colonization resistance against non-typhoidal Salmonella during concurrent malaria parasite infection. Scientific Reports, 5, [14603]. https://doi.org/10.1038/srep14603