Infection of cultured rat hepatoma cells by mouse mammary tumor virus

Gordon M. Ringold, Robert D. Cardiff, Harold E. Varmus, Keith R. Yamamoto

Research output: Contribution to journalArticlepeer-review

39 Scopus citations


A continuous line of buffalo rat hepatoma (HTC) cells has been successfully infected with mouse mammary tumor virus (MMTV) produced by the GR mammary tumor cell line. Uniform infection required initial exposure of the HTC cells to >105 MMTV particles per cell. The resultant chronically infected cell population was found to have stably acquired 20-30 copies of MMTV DNA. The infected cells contain viral RNA and express viral antigens; however, very few MMTV particles are released into the culture medium. In spite of the biochemical evidence for infection, we have not detected any alterations in the morphology or growth properties of the infected HTC cells. As is the case in mammary tumor cells, the intracellular concentration of viral RNA is strongly stimulated (50-150 fold) by the synthetic glucocorticoid, dexamethasone. Thus it appears that the mechanisms by which glucocorticoids regulate MMTV gene expression in mouse cells are maintained when this virus infects nonmurine cells.

Original languageEnglish (US)
Pages (from-to)11-18
Number of pages8
Issue number1
StatePublished - Jan 1 1977
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)


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