Infection of bovine fetuses at 120 days' gestation with virulent and avirulent strains of bluetongue virus serotype 11

A. S. Waldvogel, G. A. Anderson, D. L. Phillips, Bennie Osburn

Research output: Contribution to journalReview article

16 Citations (Scopus)

Abstract

Bluetongue virus infection in sheep and cattle during fetal development causes neuropathology. Two strains of bluetongue virus serotype 11 designated as UC-2 and UC-8 have different virulence patterns in newborn mice. These viruses have distinctly different electropherotype patterns on polyacrylamide gel electrophoresis indicating a genetic difference in these two viruses of the same serotype. Four bovine fetuses each were inoculated intramuscularly with either UC-2 or UC-8, and one fetus was inoculated with placebo. The inoculation was made intramuscularly through the uterine wall at 120 days' gestation, and the bovine fetuses were recovered by cesarean section 12 or 20 days after inoculation. Fetal blood was collected for virus isolation and serology. Virus was reisolated from brain, blood, lung and liver. Both strains, UC-2 and UC-8, cause severe lesions in the 120 day fetuses. The encephalomalacic lesions occurred earlier and were more severe in fetuses inoculated with UC-8 as compared to those inoculated with UC-2. The subtle differences observed in the fetuses inoculated with the two different strains suggest that there is a difference in pathogenic potential of the two viruses. These differences do not appear to be completely dependent upon the host species.

Original languageEnglish (US)
Pages (from-to)53-63
Number of pages11
JournalComparative Immunology, Microbiology and Infectious Diseases
Volume15
Issue number1
DOIs
StatePublished - Jan 1 1992

Fingerprint

Bluetongue virus
avirulent strains
fetus
serotypes
Fetus
pregnancy
Pregnancy
Viruses
cattle
viruses
Infection
infection
lesions (animal)
neuropathology
cesarean section
blood
fetal development
Virus Diseases
Serology
Fetal Development

Keywords

  • bluetongue virus
  • bovine
  • cattle
  • fetal
  • neuropathology

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Immunology
  • Microbiology
  • veterinary(all)

Cite this

Infection of bovine fetuses at 120 days' gestation with virulent and avirulent strains of bluetongue virus serotype 11. / Waldvogel, A. S.; Anderson, G. A.; Phillips, D. L.; Osburn, Bennie.

In: Comparative Immunology, Microbiology and Infectious Diseases, Vol. 15, No. 1, 01.01.1992, p. 53-63.

Research output: Contribution to journalReview article

@article{be485233dcb548d58c08293f72417bb6,
title = "Infection of bovine fetuses at 120 days' gestation with virulent and avirulent strains of bluetongue virus serotype 11",
abstract = "Bluetongue virus infection in sheep and cattle during fetal development causes neuropathology. Two strains of bluetongue virus serotype 11 designated as UC-2 and UC-8 have different virulence patterns in newborn mice. These viruses have distinctly different electropherotype patterns on polyacrylamide gel electrophoresis indicating a genetic difference in these two viruses of the same serotype. Four bovine fetuses each were inoculated intramuscularly with either UC-2 or UC-8, and one fetus was inoculated with placebo. The inoculation was made intramuscularly through the uterine wall at 120 days' gestation, and the bovine fetuses were recovered by cesarean section 12 or 20 days after inoculation. Fetal blood was collected for virus isolation and serology. Virus was reisolated from brain, blood, lung and liver. Both strains, UC-2 and UC-8, cause severe lesions in the 120 day fetuses. The encephalomalacic lesions occurred earlier and were more severe in fetuses inoculated with UC-8 as compared to those inoculated with UC-2. The subtle differences observed in the fetuses inoculated with the two different strains suggest that there is a difference in pathogenic potential of the two viruses. These differences do not appear to be completely dependent upon the host species.",
keywords = "bluetongue virus, bovine, cattle, fetal, neuropathology",
author = "Waldvogel, {A. S.} and Anderson, {G. A.} and Phillips, {D. L.} and Bennie Osburn",
year = "1992",
month = "1",
day = "1",
doi = "10.1016/0147-9571(92)90102-W",
language = "English (US)",
volume = "15",
pages = "53--63",
journal = "Comparative Immunology, Microbiology and Infectious Diseases",
issn = "0147-9571",
publisher = "Elsevier Limited",
number = "1",

}

TY - JOUR

T1 - Infection of bovine fetuses at 120 days' gestation with virulent and avirulent strains of bluetongue virus serotype 11

AU - Waldvogel, A. S.

AU - Anderson, G. A.

AU - Phillips, D. L.

AU - Osburn, Bennie

PY - 1992/1/1

Y1 - 1992/1/1

N2 - Bluetongue virus infection in sheep and cattle during fetal development causes neuropathology. Two strains of bluetongue virus serotype 11 designated as UC-2 and UC-8 have different virulence patterns in newborn mice. These viruses have distinctly different electropherotype patterns on polyacrylamide gel electrophoresis indicating a genetic difference in these two viruses of the same serotype. Four bovine fetuses each were inoculated intramuscularly with either UC-2 or UC-8, and one fetus was inoculated with placebo. The inoculation was made intramuscularly through the uterine wall at 120 days' gestation, and the bovine fetuses were recovered by cesarean section 12 or 20 days after inoculation. Fetal blood was collected for virus isolation and serology. Virus was reisolated from brain, blood, lung and liver. Both strains, UC-2 and UC-8, cause severe lesions in the 120 day fetuses. The encephalomalacic lesions occurred earlier and were more severe in fetuses inoculated with UC-8 as compared to those inoculated with UC-2. The subtle differences observed in the fetuses inoculated with the two different strains suggest that there is a difference in pathogenic potential of the two viruses. These differences do not appear to be completely dependent upon the host species.

AB - Bluetongue virus infection in sheep and cattle during fetal development causes neuropathology. Two strains of bluetongue virus serotype 11 designated as UC-2 and UC-8 have different virulence patterns in newborn mice. These viruses have distinctly different electropherotype patterns on polyacrylamide gel electrophoresis indicating a genetic difference in these two viruses of the same serotype. Four bovine fetuses each were inoculated intramuscularly with either UC-2 or UC-8, and one fetus was inoculated with placebo. The inoculation was made intramuscularly through the uterine wall at 120 days' gestation, and the bovine fetuses were recovered by cesarean section 12 or 20 days after inoculation. Fetal blood was collected for virus isolation and serology. Virus was reisolated from brain, blood, lung and liver. Both strains, UC-2 and UC-8, cause severe lesions in the 120 day fetuses. The encephalomalacic lesions occurred earlier and were more severe in fetuses inoculated with UC-8 as compared to those inoculated with UC-2. The subtle differences observed in the fetuses inoculated with the two different strains suggest that there is a difference in pathogenic potential of the two viruses. These differences do not appear to be completely dependent upon the host species.

KW - bluetongue virus

KW - bovine

KW - cattle

KW - fetal

KW - neuropathology

UR - http://www.scopus.com/inward/record.url?scp=0026556688&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026556688&partnerID=8YFLogxK

U2 - 10.1016/0147-9571(92)90102-W

DO - 10.1016/0147-9571(92)90102-W

M3 - Review article

C2 - 1312421

AN - SCOPUS:0026556688

VL - 15

SP - 53

EP - 63

JO - Comparative Immunology, Microbiology and Infectious Diseases

JF - Comparative Immunology, Microbiology and Infectious Diseases

SN - 0147-9571

IS - 1

ER -