Infection-induced myelopoiesis during intracellular bacterial infection is critically dependent upon IFN-γ signaling

Katherine C. MacNamara, Kwadwo Oduro, Olga Martin, Derek D. Jones, Maura McLaughlin, Kyunghee Choi, Dori L Borjesson, Gary M. Winslow

Research output: Contribution to journalArticle

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Abstract

Although microbial infections can alter steady-state hematopoiesis, the mechanisms that drive such changes are not well understood. We addressed a role for IFN-g signaling in infection-induced bone marrow suppression and anemia in a murine model of human monocytic ehrlichiosis, an emerging tick-borne disease. Within the bone marrow of Ehrlichia muris-infected C57BL/6 mice, we observed a reduction in myeloid progenitor cells, as defined both phenotypically and functionally. Infected mice exhibited a concomitant increase in developing myeloid cells within the bone marrow, an increase in the frequency of circulating monocytes, and an increase in splenic myeloid cells. The infection-induced changes in progenitor cell phenotype were critically dependent on IFN-γ, but not IFN-α, signaling. In mice deficient in the IFN-γ signaling pathway, we observed an increase in myeloid progenitor cells and CDllbloGr1lo promyelocytic cells within the bone marrow, as well as reduced frequencies of mature granulocytes and monocytes. Furthermore, E. muris-infected IFN-γR-deficient mice did not exhibit anemia or an increase in circulating monocytes, and they succumbed to infection. Gene transcription studies revealed that IFN-γR-deficient CDllb loGr1lo promyelocytes from E. muris-infected mice exhibited significantly reduced expression of irf-1 and irf-8, both key transcription factors that regulate the differentiation of granulocytes and monocytes. Finally, using mixed bone marrow chimeric mice, we show that IFN-γ-dependent infection-induced myelopoiesis occurs via the direct effect of the cytokine on developing myeloid cells. We propose that, in addition to its many other known roles, IFN-γ acts to control infection by directly promoting the differentiation of myeloid cells that contribute to host defense. Copyright

Original languageEnglish (US)
Pages (from-to)1032-1043
Number of pages12
JournalJournal of Immunology
Volume186
Issue number2
DOIs
StatePublished - Jan 15 2011

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Myelopoiesis
Bacterial Infections
Myeloid Cells
Monocytes
Bone Marrow
Myeloid Progenitor Cells
Infection
Granulocytes
Anemia
Ehrlichia
Ehrlichiosis
Tick-Borne Diseases
Granulocyte Precursor Cells
Hematopoiesis
Infection Control
Inbred C57BL Mouse
Bone Marrow Cells
Transcription Factors
Stem Cells
Cytokines

ASJC Scopus subject areas

  • Immunology

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MacNamara, K. C., Oduro, K., Martin, O., Jones, D. D., McLaughlin, M., Choi, K., ... Winslow, G. M. (2011). Infection-induced myelopoiesis during intracellular bacterial infection is critically dependent upon IFN-γ signaling. Journal of Immunology, 186(2), 1032-1043. https://doi.org/10.4049/jimmunol.1001893

Infection-induced myelopoiesis during intracellular bacterial infection is critically dependent upon IFN-γ signaling. / MacNamara, Katherine C.; Oduro, Kwadwo; Martin, Olga; Jones, Derek D.; McLaughlin, Maura; Choi, Kyunghee; Borjesson, Dori L; Winslow, Gary M.

In: Journal of Immunology, Vol. 186, No. 2, 15.01.2011, p. 1032-1043.

Research output: Contribution to journalArticle

MacNamara, Katherine C. ; Oduro, Kwadwo ; Martin, Olga ; Jones, Derek D. ; McLaughlin, Maura ; Choi, Kyunghee ; Borjesson, Dori L ; Winslow, Gary M. / Infection-induced myelopoiesis during intracellular bacterial infection is critically dependent upon IFN-γ signaling. In: Journal of Immunology. 2011 ; Vol. 186, No. 2. pp. 1032-1043.
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