Inescapable shock reduces [3H]Ro 5-4864 binding to "peripheral-type" benzodiazepine receptors in the rat

Robert C. Drugan, Anthony S. Basile, Jacqueline Crawley, Steven M. Paul, Phil Skolnick

Research output: Contribution to journalArticle

95 Citations (Scopus)

Abstract

[3H]Ro 5-4864 binding to "peripheral-type" benzodiazepine receptors was examined in brain and peripheral tissues of rats subjected to inescapable tailshocks. Two hours after a session of 80 (five-second) inescapable tailshocks, a significant reduction in [3H]Ro 5-4864 (10 nM) binding was observed in membranes from kidney (31%), cerebral cortex (29%), heart (19%) and pituitary (17%) compared to tissues from naive animals. In contrast, inescapable shock did not effect [3H]Ro 5-4864 binding to hippocampal, lung, or adrenal membranes. Scatchard analyses of [3H]Ro 5-4864 binding to renal membranes demonstrated that this session of tailshock reduced the density (Bmax) of "peripheral-type" benzodiazepine receptors without effecting the apparent affinity (Kd) of the radioligand for these sites. The effects of graded stress on [3H]Ro 5-4864 binding to cerebral cortex and kidney were investigated using 5, 20, or 80 (five-second) inescapable shocks. In cerebral cortical membranes, sessions of either 5 or 20 shocks did not affect, while 80 shocks reduced (29%) [3H]Ro 5-4864 (10 nM) binding. In renal membranes, 5 shocks significantly increased (35%), 80 shocks significantly decreased [3H]Ro 5-4864 (10 nM) binding (31%). These findings demonstrate that the density of "peripheral-type" benzodiazepine receptors in both peripheral tissues and the central nervous system can be rapidly modulated by stress.

Original languageEnglish (US)
Pages (from-to)1673-1677
Number of pages5
JournalPharmacology, Biochemistry and Behavior
Volume24
Issue number6
DOIs
StatePublished - 1986
Externally publishedYes

Fingerprint

GABA-A Receptors
Rats
Shock
Membranes
Kidney
Tissue
Cerebral Cortex
Neurology
4'-chlorodiazepam
rat Tspo protein
Brain
Animals
Central Nervous System
Lung

Keywords

  • "peripheral-type" benzodiazepine receptors
  • Inescapable shock
  • PK 11195
  • Ro 5-4864
  • Stress

ASJC Scopus subject areas

  • Biochemistry
  • Behavioral Neuroscience
  • Pharmacology

Cite this

Inescapable shock reduces [3H]Ro 5-4864 binding to "peripheral-type" benzodiazepine receptors in the rat. / Drugan, Robert C.; Basile, Anthony S.; Crawley, Jacqueline; Paul, Steven M.; Skolnick, Phil.

In: Pharmacology, Biochemistry and Behavior, Vol. 24, No. 6, 1986, p. 1673-1677.

Research output: Contribution to journalArticle

Drugan, Robert C. ; Basile, Anthony S. ; Crawley, Jacqueline ; Paul, Steven M. ; Skolnick, Phil. / Inescapable shock reduces [3H]Ro 5-4864 binding to "peripheral-type" benzodiazepine receptors in the rat. In: Pharmacology, Biochemistry and Behavior. 1986 ; Vol. 24, No. 6. pp. 1673-1677.
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