Induction of Th2 cytokine expression for p27-specific IgA B cell responses after targeted lymph node immunization with simian immunodeficiency virus antigens in rhesus macaques

Shigetada Kawabata, Chris J Miller, Thomas Lehner, Kohtaro Fujihashi, Mitsuru Kubota, Jerry R. McGhee, Koichi Imaoka, Takachika Hiroi, Hiroshi Kiyono

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

To determine if there is an association between the isotype of simian immunodeficiency virus (SIV)-specific B cell responses and the profile of Th1 and Th2 cytokine expression, rhesus macaques were immunized with SIV antigens via the iliac lymph nodes, using a targeted lymph node (TLN) immunization procedure. When CD4+ T cells purified from antigen-stimulated peripheral blood mononuclear cells were analyzed, the levels of Th2 cytokine production were gradually increased after the second and third immunizations. However, interferon-γ production did not change. Analysis of SIV-specific B cell responses revealed that the main isotype was IgG after the second and third immunizations. In addition, a peak of SIV-specific IgA B cell responses was noted following the third immunization. These findings suggest that the induction of Th2 type responses in TLN-immunized rhesus macaques reflects the sequence of initial induction of SIV-specific IgG-producing cells followed by IgA-secreting cells.

Original languageEnglish (US)
Pages (from-to)26-33
Number of pages8
JournalJournal of Infectious Diseases
Volume177
Issue number1
StatePublished - 1998
Externally publishedYes

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Immunology

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