Induction of stress proteins and MMP-9 by 0.8 ppm of ozone in murine skin

Giuseppe Valacchi, Elisa Pagnin, Tatsuya Okamoto, Ana M. Corbacho, Estibaliz Olano, Paul A. Davis, Albert Van Der Vliet, Lester Packer, Carroll E Cross

Research output: Contribution to journalArticlepeer-review

55 Scopus citations


Ozone (O3) is among the most reactive environmental oxidant pollutants to which cutaneous tissues are exposed. O3 exposure has been shown to induce antioxidant depletion as well as the oxidation of lipids and proteins within the outermost skin layer, the stratum corneum. However, relatively little is known regarding the potential effects of O3 on the cellular constituents of the underlying skin epidermis and dermis. In the present study, hairless mice exposed for 6h to 0.8ppm O3 showed increases in lipid peroxidation, as quantitated by increases in 4-hydroxynonenal-protein adducts. O3 exposure caused an induction of the stress proteins HSP27 and heme oxygenase-1 (HO-1), starting at 6h and increasing up to 18h after O3 exposure. This was accompanied by an increase in matrix metalloproteinase-9 (MMP-9) mRNA and activity levels, indicative of possible injurious-reparative processes. Collectively, our data demonstrate that skin exposure to O3 not only affects antioxidant levels and oxidation markers in the outermost stratum corneum layer, but also induces cellular stress responses in the deeper cellular layers of the skin.

Original languageEnglish (US)
Pages (from-to)741-746
Number of pages6
JournalBiochemical and Biophysical Research Communications
Issue number3
StatePublished - Jun 6 2003


  • Gelatinases
  • Heat stress proteins
  • Heme oxygenase
  • Hydroxynonenal
  • Keratinocytes
  • Lipid peroxidation
  • Metalloproteinases
  • Ozone

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology


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