Induction of simian acquired immune deficiency syndrome (SAIDS) with a molecular clone of a type D SAIDS retrovirus

G. Heidecker, N. W. Lerche, Linda J Lowenstine, A. A. Lackner, K. G. Osborn, M. B. Gardner, P. A. Marx

Research output: Contribution to journalArticle

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Abstract

We have isolated a molecular clone of the full-length integrated provirus of simian acquired immune deficiency syndrome retrovirus serotype 1 (SRV-1) from a fatal case of simian acquired immune deficiency syndrome in a juvenile rhesus macaque. An integrated SRV-1 provirus was cloned, sequenced, and found to contain four large open reading frames encoding gag-precursor protein, protease, polymerase, and envelope. The proviral clone was transfected into D17 canine osteosarcoma cells and found to produce infectious virus. A comparison of the sequences of this clone with a noninfectious clone showed 20 differences, resulting in 10 amino acid changes. Also, a cluster of exchanges, short insertions, and deletions in the 5' leader sequences resulted in extension of the tRNA(Lys) primer-binding site from 14 to 19 nucleotides. Virus isolated from transfected cells was shown to be infectious and pathogenic, resulting in disease that followed the same time course and mortality as disease induced by uncloned, in vitro cultivated virus isolated from diseased animals. These results unequivocally show that a type D retrovirus (SRV-1) causes a fatal immunosuppressive syndrome in rhesus monkeys.

Original languageEnglish (US)
Pages (from-to)3066-3071
Number of pages6
JournalJournal of Virology
Volume61
Issue number10
StatePublished - 1987
Externally publishedYes

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Simian Acquired Immunodeficiency Syndrome
Retroviridae
acquired immunodeficiency syndrome
Clone Cells
clones
proviruses
Proviruses
serotypes
Macaca mulatta
viruses
Betaretrovirus
RNA, Transfer, Lys
Viruses
gag Gene Products
immunosuppressive agents
osteosarcoma
5' untranslated regions
Virus Diseases
Osteosarcoma
Immunosuppressive Agents

ASJC Scopus subject areas

  • Immunology

Cite this

Heidecker, G., Lerche, N. W., Lowenstine, L. J., Lackner, A. A., Osborn, K. G., Gardner, M. B., & Marx, P. A. (1987). Induction of simian acquired immune deficiency syndrome (SAIDS) with a molecular clone of a type D SAIDS retrovirus. Journal of Virology, 61(10), 3066-3071.

Induction of simian acquired immune deficiency syndrome (SAIDS) with a molecular clone of a type D SAIDS retrovirus. / Heidecker, G.; Lerche, N. W.; Lowenstine, Linda J; Lackner, A. A.; Osborn, K. G.; Gardner, M. B.; Marx, P. A.

In: Journal of Virology, Vol. 61, No. 10, 1987, p. 3066-3071.

Research output: Contribution to journalArticle

Heidecker, G, Lerche, NW, Lowenstine, LJ, Lackner, AA, Osborn, KG, Gardner, MB & Marx, PA 1987, 'Induction of simian acquired immune deficiency syndrome (SAIDS) with a molecular clone of a type D SAIDS retrovirus', Journal of Virology, vol. 61, no. 10, pp. 3066-3071.
Heidecker, G. ; Lerche, N. W. ; Lowenstine, Linda J ; Lackner, A. A. ; Osborn, K. G. ; Gardner, M. B. ; Marx, P. A. / Induction of simian acquired immune deficiency syndrome (SAIDS) with a molecular clone of a type D SAIDS retrovirus. In: Journal of Virology. 1987 ; Vol. 61, No. 10. pp. 3066-3071.
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