Induction of severe experimental autoimmune neuritis with a synthetic peptide corresponding to the 53-78 amino acid sequence of the myelin P2 protein

Abdolmohamad Rostami, Shahik K. Gregorian, Mark J. Brown, David E Pleasure

Research output: Contribution to journalArticle

78 Scopus citations

Abstract

We generated a synthetic peptide (SP-26), corresponding to the amino acid residues 53-78 of bovine P2 protein, which induced severe clinical and pathological characteristics of experimental autoimmune neuritis (EAN) in Lewis rats. Lymph node cell populations from SP-26-immunized rats elicited a proliferative response to the peptide and to the P2 protein. After 16 cycles of antigen stimulation with the peptide, the SP-26 T cell line shows a decreased response to P2, but not to SP-26. Fluorescence-activated cell sorter (FACS) analysis of a SP-26 T cell line indicated the majority of cells to be of CD4+ CD8-. This report demonstrates that the synthetic peptide SP-26 can induce severe EAN in Lewis rats in a dose-dependent manner. Furthermore, specific T cell lines reactive to SP-26 can be generated from the lymph nodes of SP-26-immunized rats.

Original languageEnglish (US)
Pages (from-to)145-151
Number of pages7
JournalJournal of Neuroimmunology
Volume30
Issue number2-3
DOIs
StatePublished - 1990
Externally publishedYes

Keywords

  • (Lewis rat)
  • Experimental autoimmune neuritis
  • SP-26-specific T cell line
  • T cell epitope

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Clinical Neurology
  • Neurology

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