Induction of NO synthase 2 in ventricular cardiomyocytes incubated with a conventional bicarbonate dialysis bath

Eleonora Grandi, Marco Govoni, Simone Furini, Stefano Severi, Emanuele Giordano, Antonio Santoro, Silvio Cavalcanti

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Background. In hypotension-prone patients, conventional bicarbonate dialysis (BD) causes a reduced cardiovascular tolerance to the treatment with respect to acetate-free biofiltration (AFB). One possible explanation is an overproduction of endogenous NO (nitric oxide) due to the residual quote of acetate (4 mM) in the BD dialysate formulation. NO overload might cause the impairment of cardiovascular reactivity observed during BD. In this study, a potential direct impact of the residual quote of acetate on the cardiac cells was investigated. Methods. Ventricular cardiac myocytes isolated from adult rat hearts were treated with three different dialysis baths with or without acetate: BD, AFB and AFB + 4 mM of acetate (AFB+). Corresponding levels of expression of the inducible NO synthase 2 (NOS2) were assessed after the treatments along with the measurement of single-cell action potential (AP). Results. Incubation with acetate-containing dialysis solutions significantly enhanced (P < 0.05) the expression of NOS2 mRNA (BD: 1.11 ± 0.31; AFB+: 0.73 ± 0.04, NOS2/GAPDH intensitometric ratio) with respect to the acetate-free bath (AFB: 0.03 ± 0.01). Accordingly, protein translation was also enhanced (BD: 0.176 ± 0.021; AFB+: 0.135 ± 0.009, NOS2/α-tubuline intensitometric ratio) with respect to AFB (0.002 ± 0.001, P < 0.05). Measurement of the AP indicates that acetate-containing solutions determine a shortening of the repolarization phase as compared to treatment with AFB (BD: 95 ± 13; AFB+: 76 ± 10; AFB: 162 ± 16 ms). Conclusion. These findings show that the residual quote of acetate of the BD bath formulation affects the expression of NOS2 and the duration of AP in cardiac cells. This might cause the cardiac contractile impairment in unstable patients during BD treatment.

Original languageEnglish (US)
Pages (from-to)2192-2197
Number of pages6
JournalNephrology Dialysis Transplantation
Volume23
Issue number7
DOIs
StatePublished - Jul 1 2008
Externally publishedYes

Fingerprint

Hemodiafiltration
Bicarbonates
Baths
Cardiac Myocytes
Nitric Oxide Synthase
Dialysis
Acetates
Action Potentials
Dialysis Solutions
Nitric Oxide
Protein Biosynthesis
Nitric Oxide Synthase Type II
Therapeutics
Hypotension

Keywords

  • Acetate
  • Cardiomyocyte
  • Haemodialysis
  • Hypotension
  • Nitric oxide

ASJC Scopus subject areas

  • Nephrology
  • Transplantation

Cite this

Induction of NO synthase 2 in ventricular cardiomyocytes incubated with a conventional bicarbonate dialysis bath. / Grandi, Eleonora; Govoni, Marco; Furini, Simone; Severi, Stefano; Giordano, Emanuele; Santoro, Antonio; Cavalcanti, Silvio.

In: Nephrology Dialysis Transplantation, Vol. 23, No. 7, 01.07.2008, p. 2192-2197.

Research output: Contribution to journalArticle

Grandi, Eleonora ; Govoni, Marco ; Furini, Simone ; Severi, Stefano ; Giordano, Emanuele ; Santoro, Antonio ; Cavalcanti, Silvio. / Induction of NO synthase 2 in ventricular cardiomyocytes incubated with a conventional bicarbonate dialysis bath. In: Nephrology Dialysis Transplantation. 2008 ; Vol. 23, No. 7. pp. 2192-2197.
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AU - Furini, Simone

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AU - Giordano, Emanuele

AU - Santoro, Antonio

AU - Cavalcanti, Silvio

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N2 - Background. In hypotension-prone patients, conventional bicarbonate dialysis (BD) causes a reduced cardiovascular tolerance to the treatment with respect to acetate-free biofiltration (AFB). One possible explanation is an overproduction of endogenous NO (nitric oxide) due to the residual quote of acetate (4 mM) in the BD dialysate formulation. NO overload might cause the impairment of cardiovascular reactivity observed during BD. In this study, a potential direct impact of the residual quote of acetate on the cardiac cells was investigated. Methods. Ventricular cardiac myocytes isolated from adult rat hearts were treated with three different dialysis baths with or without acetate: BD, AFB and AFB + 4 mM of acetate (AFB+). Corresponding levels of expression of the inducible NO synthase 2 (NOS2) were assessed after the treatments along with the measurement of single-cell action potential (AP). Results. Incubation with acetate-containing dialysis solutions significantly enhanced (P < 0.05) the expression of NOS2 mRNA (BD: 1.11 ± 0.31; AFB+: 0.73 ± 0.04, NOS2/GAPDH intensitometric ratio) with respect to the acetate-free bath (AFB: 0.03 ± 0.01). Accordingly, protein translation was also enhanced (BD: 0.176 ± 0.021; AFB+: 0.135 ± 0.009, NOS2/α-tubuline intensitometric ratio) with respect to AFB (0.002 ± 0.001, P < 0.05). Measurement of the AP indicates that acetate-containing solutions determine a shortening of the repolarization phase as compared to treatment with AFB (BD: 95 ± 13; AFB+: 76 ± 10; AFB: 162 ± 16 ms). Conclusion. These findings show that the residual quote of acetate of the BD bath formulation affects the expression of NOS2 and the duration of AP in cardiac cells. This might cause the cardiac contractile impairment in unstable patients during BD treatment.

AB - Background. In hypotension-prone patients, conventional bicarbonate dialysis (BD) causes a reduced cardiovascular tolerance to the treatment with respect to acetate-free biofiltration (AFB). One possible explanation is an overproduction of endogenous NO (nitric oxide) due to the residual quote of acetate (4 mM) in the BD dialysate formulation. NO overload might cause the impairment of cardiovascular reactivity observed during BD. In this study, a potential direct impact of the residual quote of acetate on the cardiac cells was investigated. Methods. Ventricular cardiac myocytes isolated from adult rat hearts were treated with three different dialysis baths with or without acetate: BD, AFB and AFB + 4 mM of acetate (AFB+). Corresponding levels of expression of the inducible NO synthase 2 (NOS2) were assessed after the treatments along with the measurement of single-cell action potential (AP). Results. Incubation with acetate-containing dialysis solutions significantly enhanced (P < 0.05) the expression of NOS2 mRNA (BD: 1.11 ± 0.31; AFB+: 0.73 ± 0.04, NOS2/GAPDH intensitometric ratio) with respect to the acetate-free bath (AFB: 0.03 ± 0.01). Accordingly, protein translation was also enhanced (BD: 0.176 ± 0.021; AFB+: 0.135 ± 0.009, NOS2/α-tubuline intensitometric ratio) with respect to AFB (0.002 ± 0.001, P < 0.05). Measurement of the AP indicates that acetate-containing solutions determine a shortening of the repolarization phase as compared to treatment with AFB (BD: 95 ± 13; AFB+: 76 ± 10; AFB: 162 ± 16 ms). Conclusion. These findings show that the residual quote of acetate of the BD bath formulation affects the expression of NOS2 and the duration of AP in cardiac cells. This might cause the cardiac contractile impairment in unstable patients during BD treatment.

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