Induction of NGFI-A mRNA following middle cerebral artery occlusion in rats: In situ hybridization study

Hiroyuki Kinouchi, Frank R Sharp, Pak H. Chan, Jari Koistinaho, Stephen M. Sagar, Takashi Yoshimoto

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Middle cerebral artery (MCA) occlusion in halothane-anesthetized rats induced the zinc finger gene, NGFI-A, in brain. In situ hybridization studies showed that NGFI-A was induced throughout all of the cortex following MCA occlusion. By 24 h after MCA occlusion there was little expression of NGFI-A mRNA in the core of the MCA infarct, but the mRNA was still induced in all of cortex outside the infarct. MCA occlusion also induced this gene in subcortical structures: ipsilateral medial striatum; most of thalamus including medial and lateral geniculate nuclei; substantia nigra; and hippocampus at 4 h of MCA occlusion which generally disappeared by 24 h of MCA occlusion. Most of these structures, except for the striatum, are not supplied by the MCA. These data show that changes in brain gene expression can occur in many regions remote from an infarction.

Original languageEnglish (US)
Pages (from-to)163-166
Number of pages4
JournalNeuroscience Letters
Volume171
Issue number1-2
DOIs
StatePublished - Apr 25 1994
Externally publishedYes

Fingerprint

Middle Cerebral Artery Infarction
In Situ Hybridization
Messenger RNA
Geniculate Bodies
Middle Cerebral Artery
Zinc Fingers
Brain
Halothane
Substantia Nigra
Thalamus
Infarction
Genes
Hippocampus
Gene Expression

Keywords

  • Central nervous system
  • Immediate early gene
  • Ischemia
  • Stroke
  • Zinc finger

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Induction of NGFI-A mRNA following middle cerebral artery occlusion in rats : In situ hybridization study. / Kinouchi, Hiroyuki; Sharp, Frank R; Chan, Pak H.; Koistinaho, Jari; Sagar, Stephen M.; Yoshimoto, Takashi.

In: Neuroscience Letters, Vol. 171, No. 1-2, 25.04.1994, p. 163-166.

Research output: Contribution to journalArticle

Kinouchi, Hiroyuki ; Sharp, Frank R ; Chan, Pak H. ; Koistinaho, Jari ; Sagar, Stephen M. ; Yoshimoto, Takashi. / Induction of NGFI-A mRNA following middle cerebral artery occlusion in rats : In situ hybridization study. In: Neuroscience Letters. 1994 ; Vol. 171, No. 1-2. pp. 163-166.
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