Induction of murine AIDS virus-related sequences after burn injury

Kiho Cho, Lee K. Adamson, David G Greenhalgh

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

To better understand the molecular signaling events leading to systemic inflammatory response syndrome (SIRS) and multiple organ failure (MOF), changes in gene expression profiles after burn injury were investigated by differential display. C57BLKS/J mice were subjected to 18% total body surface area (TBSA) full-thickness burn and various tissues were harvested at multiple time points after injury. Initial differential display revealed that retroviral transcripts similar to the envelope sequence of murine AIDS (MAIDS) virus were rapidly and transiently up-regulated after injury. Subsequent RT-PCR and DNA sequencing analyses confirmed the transient up-regulation of retroviral sequences similar to those of the MAIDS virus. In addition, the presence and induction of the subgenomic envelope transcripts of these MAIDS virus-related sequences, including a novel double spliced message, were identified after burn injury. These data suggest that the transcriptional efficiency of the integrated retroviral DNA and reactivation of defective MAIDS virus-related sequences may be affected by pathophysiological signals, such as burn injury. The elevated expression of these MAIDS virus-related retroviral sequences may affect the transcriptional activities of the flanking genes at the integration sites and may be a cause of altered local and systemic immune responses to burn-related stress.

Original languageEnglish (US)
Pages (from-to)53-62
Number of pages10
JournalJournal of Surgical Research
Volume104
Issue number1
DOIs
StatePublished - 2002

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Murine Acquired Immunodeficiency Syndrome
HIV
Wounds and Injuries
Systemic Inflammatory Response Syndrome
Multiple Organ Failure
Body Surface Area
Burns
DNA Sequence Analysis
Transcriptome
Up-Regulation
Polymerase Chain Reaction
DNA
Genes

Keywords

  • Burn
  • Injury
  • MuLV
  • Murine AIDS
  • Systemic response

ASJC Scopus subject areas

  • Surgery

Cite this

Induction of murine AIDS virus-related sequences after burn injury. / Cho, Kiho; Adamson, Lee K.; Greenhalgh, David G.

In: Journal of Surgical Research, Vol. 104, No. 1, 2002, p. 53-62.

Research output: Contribution to journalArticle

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