Induction of monocyte- and T-cell-attracting chemokines in the lung during the generation of idiopathic pneumonia syndrome following allogeneic murine bone marrow transplantation

A. Panoskaltsis-Mortari, R. M. Strieter, J. R. Hermanson, K. V. Fegeding, William J Murphy, C. L. Farrell, D. L. Lacey, B. R. Blazar

Research output: Contribution to journalArticle

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Abstract

Idiopathic pneumonia syndrome (IPS) is a significant complication following bone marrow transplantation (BMT). We have developed a murine model in which severe IPS is induced by pre-BMT conditioning and allogeneic T cells and Is characterized by the recruitment of host monocytes and donor T cells into the lung by day 7 post-BMT. Chemokines regulate cellular recruitment and the migration of cells into Inflammatory lesions. In this study, we examined the profiles of chemokines produced locally in the lung (parenchyma and bronchoalveolar lavage fluid) and systemically (serum) during the generation of IPS in the peri-BMT period. Protein and messenger RNA (mRHA) levels of CC chemokines (monocyte/lymphocyte attractants), especially monocyte chemoattractant protein (MCP)-1, macrophage inflammatory protein (MIP)1α, RANTES (regulated upon activation normal T-cell expressed and secreted), and C10, were preferentially Induced in the lung by day 7 postallogeneic BMT. In addition, there was an increase in mRNA for IP-10 (a monocyte and Th1-cell chemoattractant). The CXC chemokines MIP-2 and KC, known neutrophil attractants, were moderately elevated. For the most part, these Increases in chemokines were dependent on the coinfusion of allogeneic T cells with the BM inoculum. Ribonuclease protection assay and in situ hybridization analyses post-BMT showed that the lung was a major producer of MCP-1, a potent inducer of monocyte chemotaxis. Increases in MCP-1 levels In the lung preceded host APC influx whereas MIP-1α levels accompanied donor T-cell Infiltration. In summary, we have shown that monocyte- and T-cell-attracting chemokines are associated with monocyte and T-cell recruitment during IPS. (C) 2000 by The American Society of Hematology.

Original languageEnglish (US)
Pages (from-to)834-839
Number of pages6
JournalBlood
Volume96
Issue number3
StatePublished - Aug 1 2000
Externally publishedYes

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T-cells
Bone Marrow Transplantation
Chemokines
Monocytes
Pneumonia
Bone
T-Lymphocytes
Lung
Chemokine CCL2
Macrophage Inflammatory Proteins
Bronchoalveolar Lavage Fluid
Transplantation Conditioning
Chemokine CXCL2
CXC Chemokines
CC Chemokines
Messenger RNA
Th1 Cells
Lymphocytes
Chemotactic Factors
Chemotaxis

ASJC Scopus subject areas

  • Hematology

Cite this

Panoskaltsis-Mortari, A., Strieter, R. M., Hermanson, J. R., Fegeding, K. V., Murphy, W. J., Farrell, C. L., ... Blazar, B. R. (2000). Induction of monocyte- and T-cell-attracting chemokines in the lung during the generation of idiopathic pneumonia syndrome following allogeneic murine bone marrow transplantation. Blood, 96(3), 834-839.

Induction of monocyte- and T-cell-attracting chemokines in the lung during the generation of idiopathic pneumonia syndrome following allogeneic murine bone marrow transplantation. / Panoskaltsis-Mortari, A.; Strieter, R. M.; Hermanson, J. R.; Fegeding, K. V.; Murphy, William J; Farrell, C. L.; Lacey, D. L.; Blazar, B. R.

In: Blood, Vol. 96, No. 3, 01.08.2000, p. 834-839.

Research output: Contribution to journalArticle

Panoskaltsis-Mortari, A, Strieter, RM, Hermanson, JR, Fegeding, KV, Murphy, WJ, Farrell, CL, Lacey, DL & Blazar, BR 2000, 'Induction of monocyte- and T-cell-attracting chemokines in the lung during the generation of idiopathic pneumonia syndrome following allogeneic murine bone marrow transplantation', Blood, vol. 96, no. 3, pp. 834-839.
Panoskaltsis-Mortari, A. ; Strieter, R. M. ; Hermanson, J. R. ; Fegeding, K. V. ; Murphy, William J ; Farrell, C. L. ; Lacey, D. L. ; Blazar, B. R. / Induction of monocyte- and T-cell-attracting chemokines in the lung during the generation of idiopathic pneumonia syndrome following allogeneic murine bone marrow transplantation. In: Blood. 2000 ; Vol. 96, No. 3. pp. 834-839.
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abstract = "Idiopathic pneumonia syndrome (IPS) is a significant complication following bone marrow transplantation (BMT). We have developed a murine model in which severe IPS is induced by pre-BMT conditioning and allogeneic T cells and Is characterized by the recruitment of host monocytes and donor T cells into the lung by day 7 post-BMT. Chemokines regulate cellular recruitment and the migration of cells into Inflammatory lesions. In this study, we examined the profiles of chemokines produced locally in the lung (parenchyma and bronchoalveolar lavage fluid) and systemically (serum) during the generation of IPS in the peri-BMT period. Protein and messenger RNA (mRHA) levels of CC chemokines (monocyte/lymphocyte attractants), especially monocyte chemoattractant protein (MCP)-1, macrophage inflammatory protein (MIP)1α, RANTES (regulated upon activation normal T-cell expressed and secreted), and C10, were preferentially Induced in the lung by day 7 postallogeneic BMT. In addition, there was an increase in mRNA for IP-10 (a monocyte and Th1-cell chemoattractant). The CXC chemokines MIP-2 and KC, known neutrophil attractants, were moderately elevated. For the most part, these Increases in chemokines were dependent on the coinfusion of allogeneic T cells with the BM inoculum. Ribonuclease protection assay and in situ hybridization analyses post-BMT showed that the lung was a major producer of MCP-1, a potent inducer of monocyte chemotaxis. Increases in MCP-1 levels In the lung preceded host APC influx whereas MIP-1α levels accompanied donor T-cell Infiltration. In summary, we have shown that monocyte- and T-cell-attracting chemokines are associated with monocyte and T-cell recruitment during IPS. (C) 2000 by The American Society of Hematology.",
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T1 - Induction of monocyte- and T-cell-attracting chemokines in the lung during the generation of idiopathic pneumonia syndrome following allogeneic murine bone marrow transplantation

AU - Panoskaltsis-Mortari, A.

AU - Strieter, R. M.

AU - Hermanson, J. R.

AU - Fegeding, K. V.

AU - Murphy, William J

AU - Farrell, C. L.

AU - Lacey, D. L.

AU - Blazar, B. R.

PY - 2000/8/1

Y1 - 2000/8/1

N2 - Idiopathic pneumonia syndrome (IPS) is a significant complication following bone marrow transplantation (BMT). We have developed a murine model in which severe IPS is induced by pre-BMT conditioning and allogeneic T cells and Is characterized by the recruitment of host monocytes and donor T cells into the lung by day 7 post-BMT. Chemokines regulate cellular recruitment and the migration of cells into Inflammatory lesions. In this study, we examined the profiles of chemokines produced locally in the lung (parenchyma and bronchoalveolar lavage fluid) and systemically (serum) during the generation of IPS in the peri-BMT period. Protein and messenger RNA (mRHA) levels of CC chemokines (monocyte/lymphocyte attractants), especially monocyte chemoattractant protein (MCP)-1, macrophage inflammatory protein (MIP)1α, RANTES (regulated upon activation normal T-cell expressed and secreted), and C10, were preferentially Induced in the lung by day 7 postallogeneic BMT. In addition, there was an increase in mRNA for IP-10 (a monocyte and Th1-cell chemoattractant). The CXC chemokines MIP-2 and KC, known neutrophil attractants, were moderately elevated. For the most part, these Increases in chemokines were dependent on the coinfusion of allogeneic T cells with the BM inoculum. Ribonuclease protection assay and in situ hybridization analyses post-BMT showed that the lung was a major producer of MCP-1, a potent inducer of monocyte chemotaxis. Increases in MCP-1 levels In the lung preceded host APC influx whereas MIP-1α levels accompanied donor T-cell Infiltration. In summary, we have shown that monocyte- and T-cell-attracting chemokines are associated with monocyte and T-cell recruitment during IPS. (C) 2000 by The American Society of Hematology.

AB - Idiopathic pneumonia syndrome (IPS) is a significant complication following bone marrow transplantation (BMT). We have developed a murine model in which severe IPS is induced by pre-BMT conditioning and allogeneic T cells and Is characterized by the recruitment of host monocytes and donor T cells into the lung by day 7 post-BMT. Chemokines regulate cellular recruitment and the migration of cells into Inflammatory lesions. In this study, we examined the profiles of chemokines produced locally in the lung (parenchyma and bronchoalveolar lavage fluid) and systemically (serum) during the generation of IPS in the peri-BMT period. Protein and messenger RNA (mRHA) levels of CC chemokines (monocyte/lymphocyte attractants), especially monocyte chemoattractant protein (MCP)-1, macrophage inflammatory protein (MIP)1α, RANTES (regulated upon activation normal T-cell expressed and secreted), and C10, were preferentially Induced in the lung by day 7 postallogeneic BMT. In addition, there was an increase in mRNA for IP-10 (a monocyte and Th1-cell chemoattractant). The CXC chemokines MIP-2 and KC, known neutrophil attractants, were moderately elevated. For the most part, these Increases in chemokines were dependent on the coinfusion of allogeneic T cells with the BM inoculum. Ribonuclease protection assay and in situ hybridization analyses post-BMT showed that the lung was a major producer of MCP-1, a potent inducer of monocyte chemotaxis. Increases in MCP-1 levels In the lung preceded host APC influx whereas MIP-1α levels accompanied donor T-cell Infiltration. In summary, we have shown that monocyte- and T-cell-attracting chemokines are associated with monocyte and T-cell recruitment during IPS. (C) 2000 by The American Society of Hematology.

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