Induction of microsomal dimethylnitrosamine demethylase by pyrazole

Ritva P. Evarts, Emily Haliday, Masahiko Negishi, Leonard M Hjelmeland

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Pyrazole, a potent inhibitor of alcohol dehydrogenase, was found to be a potent inducer of the activity of low (iKm) dimethylnitrosamine demethylase (DMN-d). One injection of pyrazole (200 mg/kg body wt) to weanling Wistar rats changed the microsomal DMN demethylase activity by 1.7, 1.9 and 2.5 times the control values at 6, 12 and 24 hr after the injection respectively. Pyrazole administration reduced arylhydrocarbon hydroxylase (AHH) activity. When animals were injected with pyrazole (200 mg/kg body wt) for 1, 2, 3 or 4 consecutive days, the values for DMN-d activity were 277, 297, 306 and 319% of the control values. The corresponding values for AHH were 91, 67, 57 and 45% for l, 2, 3 and 4 injections respectively. Pyrazole-induced DMN-d activity was NADPH dependent and was inhibited by CO; n-butanol gave a 50% inhibition at a concentration of 2 × 10-3 M. The corresponding value for metyrapone was 1 × 10-2 M. Cytochrome P-450 was slightly increased by pyrazole and its CO-complex gave an absorption maximum around 451 nm. When the microsomal proteins were separated using sodium dodecylsulfate (SDS)-polyacrylamide gel electrophoresis, a large increase in a band at about 51,000 daltons was found in the liver microsomes of pyrazole-treated animals.

Original languageEnglish (US)
Pages (from-to)1245-1249
Number of pages5
JournalBiochemical Pharmacology
Volume31
Issue number7
DOIs
StatePublished - Apr 1 1982
Externally publishedYes

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Dimethylnitrosamine
Carbon Monoxide
Mixed Function Oxygenases
Injections
Animals
Metyrapone
1-Butanol
Alcohol Dehydrogenase
Liver Microsomes
pyrazole
Electrophoresis
NADP
Liver
Cytochrome P-450 Enzyme System
Wistar Rats
Rats
Polyacrylamide Gel Electrophoresis
Sodium

ASJC Scopus subject areas

  • Pharmacology

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Induction of microsomal dimethylnitrosamine demethylase by pyrazole. / Evarts, Ritva P.; Haliday, Emily; Negishi, Masahiko; Hjelmeland, Leonard M.

In: Biochemical Pharmacology, Vol. 31, No. 7, 01.04.1982, p. 1245-1249.

Research output: Contribution to journalArticle

Evarts, Ritva P. ; Haliday, Emily ; Negishi, Masahiko ; Hjelmeland, Leonard M. / Induction of microsomal dimethylnitrosamine demethylase by pyrazole. In: Biochemical Pharmacology. 1982 ; Vol. 31, No. 7. pp. 1245-1249.
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