Induction of labyrinthitis ossificans after pneumococcal meningitis: An animal model

H. A. Brodie, T. C. Thompson, L. Vassilian, B. N. Lee

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Newly formed disorganized bone fills the open spaces within the otic capsule in various pathologic conditions, resulting in labyrinthitis ossificans. The pathologic mechanisms of this disease remain poorly understood. To better study the sequence of events and contributing mechanisms involved in labyrinthitis ossificans, an animal model was developed. Three groups of Mongolian gerbils received either an intralabyrinthine injection of normal saline solution (group 1) or Streptococcus pneumoniae polysaccharide capsule antigens (groups 2 and 3). The temporal bones were harvested after 3 months and serially sectioned. None of the eight control animals (group 1), which received intralabyrinthine injections of normal saline solution had any histologic changes in their temporal bones. Nine of the surviving 19 animals in groups 2 and 3 had fibrosis or evidence of early ossffication. A fourth group of Mongolian gerbils received two intrathecal injections of live S. pneumoniae organisms. The temporal bones were harvested after 3 months and serially sectioned. Fourteen of the surviving 15 animals had fibrosis or ossification or both. This animal model will provide a method for study of the mechanisms of labyrinthitis ossificans.

Original languageEnglish (US)
Pages (from-to)15-21
Number of pages7
JournalOtolaryngology - Head and Neck Surgery
Volume118
Issue number1
DOIs
StatePublished - 1998

Fingerprint

Labyrinthitis
Pneumococcal Meningitis
Temporal Bone
Animal Models
Gerbillinae
Streptococcus pneumoniae
Sodium Chloride
Capsules
Fibrosis
Spinal Injections
Injections
Osteogenesis
Polysaccharides
Ear
Antigens
Bone and Bones
Control Groups

ASJC Scopus subject areas

  • Otorhinolaryngology

Cite this

Induction of labyrinthitis ossificans after pneumococcal meningitis : An animal model. / Brodie, H. A.; Thompson, T. C.; Vassilian, L.; Lee, B. N.

In: Otolaryngology - Head and Neck Surgery, Vol. 118, No. 1, 1998, p. 15-21.

Research output: Contribution to journalArticle

Brodie, H. A. ; Thompson, T. C. ; Vassilian, L. ; Lee, B. N. / Induction of labyrinthitis ossificans after pneumococcal meningitis : An animal model. In: Otolaryngology - Head and Neck Surgery. 1998 ; Vol. 118, No. 1. pp. 15-21.
@article{49056d5c91ab439c8b11220b8614ed84,
title = "Induction of labyrinthitis ossificans after pneumococcal meningitis: An animal model",
abstract = "Newly formed disorganized bone fills the open spaces within the otic capsule in various pathologic conditions, resulting in labyrinthitis ossificans. The pathologic mechanisms of this disease remain poorly understood. To better study the sequence of events and contributing mechanisms involved in labyrinthitis ossificans, an animal model was developed. Three groups of Mongolian gerbils received either an intralabyrinthine injection of normal saline solution (group 1) or Streptococcus pneumoniae polysaccharide capsule antigens (groups 2 and 3). The temporal bones were harvested after 3 months and serially sectioned. None of the eight control animals (group 1), which received intralabyrinthine injections of normal saline solution had any histologic changes in their temporal bones. Nine of the surviving 19 animals in groups 2 and 3 had fibrosis or evidence of early ossffication. A fourth group of Mongolian gerbils received two intrathecal injections of live S. pneumoniae organisms. The temporal bones were harvested after 3 months and serially sectioned. Fourteen of the surviving 15 animals had fibrosis or ossification or both. This animal model will provide a method for study of the mechanisms of labyrinthitis ossificans.",
author = "Brodie, {H. A.} and Thompson, {T. C.} and L. Vassilian and Lee, {B. N.}",
year = "1998",
doi = "10.1016/S0194-5998(98)70369-9",
language = "English (US)",
volume = "118",
pages = "15--21",
journal = "Otolaryngology - Head and Neck Surgery (United States)",
issn = "0194-5998",
publisher = "Mosby Inc.",
number = "1",

}

TY - JOUR

T1 - Induction of labyrinthitis ossificans after pneumococcal meningitis

T2 - An animal model

AU - Brodie, H. A.

AU - Thompson, T. C.

AU - Vassilian, L.

AU - Lee, B. N.

PY - 1998

Y1 - 1998

N2 - Newly formed disorganized bone fills the open spaces within the otic capsule in various pathologic conditions, resulting in labyrinthitis ossificans. The pathologic mechanisms of this disease remain poorly understood. To better study the sequence of events and contributing mechanisms involved in labyrinthitis ossificans, an animal model was developed. Three groups of Mongolian gerbils received either an intralabyrinthine injection of normal saline solution (group 1) or Streptococcus pneumoniae polysaccharide capsule antigens (groups 2 and 3). The temporal bones were harvested after 3 months and serially sectioned. None of the eight control animals (group 1), which received intralabyrinthine injections of normal saline solution had any histologic changes in their temporal bones. Nine of the surviving 19 animals in groups 2 and 3 had fibrosis or evidence of early ossffication. A fourth group of Mongolian gerbils received two intrathecal injections of live S. pneumoniae organisms. The temporal bones were harvested after 3 months and serially sectioned. Fourteen of the surviving 15 animals had fibrosis or ossification or both. This animal model will provide a method for study of the mechanisms of labyrinthitis ossificans.

AB - Newly formed disorganized bone fills the open spaces within the otic capsule in various pathologic conditions, resulting in labyrinthitis ossificans. The pathologic mechanisms of this disease remain poorly understood. To better study the sequence of events and contributing mechanisms involved in labyrinthitis ossificans, an animal model was developed. Three groups of Mongolian gerbils received either an intralabyrinthine injection of normal saline solution (group 1) or Streptococcus pneumoniae polysaccharide capsule antigens (groups 2 and 3). The temporal bones were harvested after 3 months and serially sectioned. None of the eight control animals (group 1), which received intralabyrinthine injections of normal saline solution had any histologic changes in their temporal bones. Nine of the surviving 19 animals in groups 2 and 3 had fibrosis or evidence of early ossffication. A fourth group of Mongolian gerbils received two intrathecal injections of live S. pneumoniae organisms. The temporal bones were harvested after 3 months and serially sectioned. Fourteen of the surviving 15 animals had fibrosis or ossification or both. This animal model will provide a method for study of the mechanisms of labyrinthitis ossificans.

UR - http://www.scopus.com/inward/record.url?scp=0031885932&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031885932&partnerID=8YFLogxK

U2 - 10.1016/S0194-5998(98)70369-9

DO - 10.1016/S0194-5998(98)70369-9

M3 - Article

C2 - 9450823

AN - SCOPUS:0031885932

VL - 118

SP - 15

EP - 21

JO - Otolaryngology - Head and Neck Surgery (United States)

JF - Otolaryngology - Head and Neck Surgery (United States)

SN - 0194-5998

IS - 1

ER -