Induction of immediate early genes by cyclic AMP in primary cultures of neurons from rat cerebral cortex

F. M. Vaccarino, M. D. Hayward, H. N. Le, Dennis Hartigan-O'Connor, R. S. Duman, E. J. Nestler

Research output: Contribution to journalArticle

43 Scopus citations

Abstract

In this paper, we tested whether physiological activators of the cAMP second messenger pathway in primary cultures of neurons from rat cerebral cortex directly induce c-fos and other immediate early gene (IEG) transcription factors. We have found that brief (30 s to 2 min) stimulation of neurons with vasoactive intestinal peptide (VIP) and SKF-38393, a D1-dopaminergic receptor agonist, potently increased mRNA levels for the IEGs c-fos, jun-B, and NGFI-A, with weaker increases for c-jun. This action was mimicked by forskolin and dibutyryl cAMP. IEG induction by VIP and dibutyryl cAMP was not blocked by excitatory amino acid receptor antagonists or by blockers of dihydropyridine-sensitive calcium channels. Moreover, calcium-free medium did not modify IEG induction by dibutyryl cAMP, suggesting that cAMP can directly regulate IEG expression in differentiated neurons independently of calcium.

Original languageEnglish (US)
Pages (from-to)76-82
Number of pages7
JournalMolecular Brain Research
Volume19
Issue number1-2
DOIs
StatePublished - 1993
Externally publishedYes

Keywords

  • c-fos
  • c-jun
  • Cerebral cortex
  • NGFI-A
  • Proto-oncogene
  • Vasoactive intestinal peptide

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience

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