Induction of heme oxygenase-1 (HO-1) in glia after traumatic brain injury

Kazumasa Fukuda, Jeremy D. Richmon, Motoki Sato, Frank R Sharp, S. Scott Panter, L. J. Noble

Research output: Contribution to journalArticlepeer-review

111 Scopus citations


In this study we examined the induction of heme oxygenase-1 (HO-1) in glia in the traumatized rat brain. HO-1 was immunolocalized in fixed sections of brain 3 h to 5 days after injury. Induction of this enzyme in astrocytes, microglia/macrophages, and oligodendrocytes was evaluated using immunofluorescent double labeling with monoclonal antibodies to glial fibrillary acidic protein, complement C3bi receptor, and myelin basic protein. Induction of HO-1 was apparent in the injured hemisphere and cerebellum as early as 24 h postinjury. The protein was likewise noted in similar regions of the brain at 72 h postinjury but appoared to be more widespread in its distribution. At 5 days postinjury, there was a notable decline in the degree of immunostaining for HO-1. HO-1 was typically induced in astrocytes in the cerebral cortex at the site of impact, in the deep cortical layers adjacent to the hemorrhagic lesions, and in the hippocampus. HO-1 was induced in Bergmann glia in the vermis of cerebellum. In addition, HO-1 was also induced in microglia/macrophages scattered throughout the ipsilateral cerebral cortex, cerebellum and subarachnoid space. These findings demonstrate prolonged glial induction of HO-1 in the traumatized brain. Such a response may reflect a protective role of these cells against secondary insults including oxidative stress.

Original languageEnglish (US)
Pages (from-to)68-75
Number of pages8
JournalBrain Research
Issue number1-2
StatePublished - Oct 14 1996
Externally publishedYes


  • astrocyte
  • Bergmann glia
  • heat shock protein
  • heme oxygenase
  • macrophage
  • microglia

ASJC Scopus subject areas

  • Neuroscience(all)


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