Induction of heme oxygenase-1 (HO-1) in glia after traumatic brain injury

Kazumasa Fukuda, Jeremy D. Richmon, Motoki Sato, Frank R Sharp, S. Scott Panter, L. J. Noble

Research output: Contribution to journalArticle

105 Citations (Scopus)

Abstract

In this study we examined the induction of heme oxygenase-1 (HO-1) in glia in the traumatized rat brain. HO-1 was immunolocalized in fixed sections of brain 3 h to 5 days after injury. Induction of this enzyme in astrocytes, microglia/macrophages, and oligodendrocytes was evaluated using immunofluorescent double labeling with monoclonal antibodies to glial fibrillary acidic protein, complement C3bi receptor, and myelin basic protein. Induction of HO-1 was apparent in the injured hemisphere and cerebellum as early as 24 h postinjury. The protein was likewise noted in similar regions of the brain at 72 h postinjury but appoared to be more widespread in its distribution. At 5 days postinjury, there was a notable decline in the degree of immunostaining for HO-1. HO-1 was typically induced in astrocytes in the cerebral cortex at the site of impact, in the deep cortical layers adjacent to the hemorrhagic lesions, and in the hippocampus. HO-1 was induced in Bergmann glia in the vermis of cerebellum. In addition, HO-1 was also induced in microglia/macrophages scattered throughout the ipsilateral cerebral cortex, cerebellum and subarachnoid space. These findings demonstrate prolonged glial induction of HO-1 in the traumatized brain. Such a response may reflect a protective role of these cells against secondary insults including oxidative stress.

Original languageEnglish (US)
Pages (from-to)68-75
Number of pages8
JournalBrain Research
Volume736
Issue number1-2
DOIs
StatePublished - Oct 14 1996
Externally publishedYes

Fingerprint

Heme Oxygenase-1
Neuroglia
Cerebellum
Brain
Microglia
Astrocytes
Cerebral Cortex
Macrophages
Complement C3b
Complement Receptors
Subarachnoid Space
Enzyme Induction
Myelin Basic Protein
Traumatic Brain Injury
Glial Fibrillary Acidic Protein
Oligodendroglia
Hippocampus
Oxidative Stress
Monoclonal Antibodies
Wounds and Injuries

Keywords

  • astrocyte
  • Bergmann glia
  • heat shock protein
  • heme oxygenase
  • macrophage
  • microglia

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Fukuda, K., Richmon, J. D., Sato, M., Sharp, F. R., Panter, S. S., & Noble, L. J. (1996). Induction of heme oxygenase-1 (HO-1) in glia after traumatic brain injury. Brain Research, 736(1-2), 68-75. https://doi.org/10.1016/0006-8993(96)00680-4

Induction of heme oxygenase-1 (HO-1) in glia after traumatic brain injury. / Fukuda, Kazumasa; Richmon, Jeremy D.; Sato, Motoki; Sharp, Frank R; Panter, S. Scott; Noble, L. J.

In: Brain Research, Vol. 736, No. 1-2, 14.10.1996, p. 68-75.

Research output: Contribution to journalArticle

Fukuda, K, Richmon, JD, Sato, M, Sharp, FR, Panter, SS & Noble, LJ 1996, 'Induction of heme oxygenase-1 (HO-1) in glia after traumatic brain injury', Brain Research, vol. 736, no. 1-2, pp. 68-75. https://doi.org/10.1016/0006-8993(96)00680-4
Fukuda, Kazumasa ; Richmon, Jeremy D. ; Sato, Motoki ; Sharp, Frank R ; Panter, S. Scott ; Noble, L. J. / Induction of heme oxygenase-1 (HO-1) in glia after traumatic brain injury. In: Brain Research. 1996 ; Vol. 736, No. 1-2. pp. 68-75.
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