Induction of heme oxygenase-1 after hyperosmotic opening of the blood- brain barrier

Jeremy D. Richmon, Kazumasa Fukuda, Nino Maida, Motoki Sato, Marcelle Bergeron, Frank R Sharp, S. Scott Panter, L. J. Noble

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

The induction of the stress protein heme oxygenase-1 (HO-1) was studied in the rat brain after intracarotid administration of hyperosmolar mannitol. HO-1 was immunolocalized in fixed sections of brain 24 h to 7 days after injection. Immunoglobulin G (IgG) was immunolocalized in adjacent sections to demonstrate areas of breakdown of the blood-brain barrier. Induction of HO-1 was also evaluated by Western immunoblots, performed at 24 h after the insult. Immunofluorescent double labelling with monoclonal antibodies to HO- 1 and either glial fibrillary acidic protein or the complement C3bi receptor was used to determine if glia/macrophages expressed HO-1. There was pronounced, widespread induction of HO-1 in the ipsilateral hemisphere and cerebellum by 24 h both by immunocytochemistry and by Western blots. This induction was markedly attenuated at later times. HO-1 was induced in astrocytes and microglia/macrophages in the ipsilateral hemisphere. In addition, the protein was induced in Bergmann glia and scattered microglia/macrophages in the cerebellum. The mechanism of induction of HO-1 in glia after opening of the blood-brain barrier could include exposure to heme proteins, denatured proteins and other plasma constituents known to induce HO-1. This glial induction may reflect a protective response of these cells.

Original languageEnglish (US)
Pages (from-to)108-118
Number of pages11
JournalBrain Research
Volume780
Issue number1
DOIs
StatePublished - Jan 5 1998
Externally publishedYes

Fingerprint

Heme Oxygenase-1
Blood-Brain Barrier
Neuroglia
Macrophages
Microglia
Cerebellum
Western Blotting
Complement C3b
Hemeproteins
Complement Receptors
Glial Fibrillary Acidic Protein
Brain
Mannitol
Heat-Shock Proteins
Astrocytes
Blood Proteins
Immunoglobulin G
Immunohistochemistry
Monoclonal Antibodies
Injections

Keywords

  • Astrocyte
  • Bergmann glia
  • Heat shock protein
  • Macrophage
  • Mannitol
  • Microglia

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Richmon, J. D., Fukuda, K., Maida, N., Sato, M., Bergeron, M., Sharp, F. R., ... Noble, L. J. (1998). Induction of heme oxygenase-1 after hyperosmotic opening of the blood- brain barrier. Brain Research, 780(1), 108-118. https://doi.org/10.1016/S0006-8993(97)01314-0

Induction of heme oxygenase-1 after hyperosmotic opening of the blood- brain barrier. / Richmon, Jeremy D.; Fukuda, Kazumasa; Maida, Nino; Sato, Motoki; Bergeron, Marcelle; Sharp, Frank R; Panter, S. Scott; Noble, L. J.

In: Brain Research, Vol. 780, No. 1, 05.01.1998, p. 108-118.

Research output: Contribution to journalArticle

Richmon, JD, Fukuda, K, Maida, N, Sato, M, Bergeron, M, Sharp, FR, Panter, SS & Noble, LJ 1998, 'Induction of heme oxygenase-1 after hyperosmotic opening of the blood- brain barrier', Brain Research, vol. 780, no. 1, pp. 108-118. https://doi.org/10.1016/S0006-8993(97)01314-0
Richmon, Jeremy D. ; Fukuda, Kazumasa ; Maida, Nino ; Sato, Motoki ; Bergeron, Marcelle ; Sharp, Frank R ; Panter, S. Scott ; Noble, L. J. / Induction of heme oxygenase-1 after hyperosmotic opening of the blood- brain barrier. In: Brain Research. 1998 ; Vol. 780, No. 1. pp. 108-118.
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