Induction of endochondral bone by demineralized bone matrix from diabetic rats

Richard Landesman, A Hari Reddi

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

In this investigation we examined the osteoinductive potential of demineralized bone matrix derived from chronically diabetic (streptozotocin-induced) rats. Long-Evans rats (28-31 days) were made diabetic with a single injection of streptozotocin (65 mg/kg) and provided food and water ad lib for 2 months. Diaphyseal shafts of femurs and tibias removed from the diabetic rats and their sibling controls were dehydrated, pulverized, sieved to 74-420 μm particles, and demineralized Matrix was then bioassayed for its ability to induce endochondral bone on day 11 following subcutaneous implantation over the thorax of Long-Evans rats. The resulting plaques of tissue were subjected to histological analysis, determination of alkaline phosphatase activity, and calcium content. Bone matrix derived from diabetic animals proved to be a significantly better inducer of endochondral bone than did control matrix.

Original languageEnglish (US)
Pages (from-to)630-634
Number of pages5
JournalCalcified Tissue International
Volume37
Issue number6
DOIs
StatePublished - Nov 1985
Externally publishedYes

Fingerprint

Bone Matrix
Long Evans Rats
Streptozocin
Bone and Bones
Tibia
Femur
Alkaline Phosphatase
Thorax
Calcium
Food
Injections
Water

Keywords

  • Diabetic bone matrix
  • Endochondral bone formation
  • Osteoinductive potential
  • Streptozotocin-induced diabetes

ASJC Scopus subject areas

  • Endocrinology
  • Orthopedics and Sports Medicine
  • Endocrinology, Diabetes and Metabolism

Cite this

Induction of endochondral bone by demineralized bone matrix from diabetic rats. / Landesman, Richard; Reddi, A Hari.

In: Calcified Tissue International, Vol. 37, No. 6, 11.1985, p. 630-634.

Research output: Contribution to journalArticle

@article{15a8bedfd37546a3b5782b3b82cc77b4,
title = "Induction of endochondral bone by demineralized bone matrix from diabetic rats",
abstract = "In this investigation we examined the osteoinductive potential of demineralized bone matrix derived from chronically diabetic (streptozotocin-induced) rats. Long-Evans rats (28-31 days) were made diabetic with a single injection of streptozotocin (65 mg/kg) and provided food and water ad lib for 2 months. Diaphyseal shafts of femurs and tibias removed from the diabetic rats and their sibling controls were dehydrated, pulverized, sieved to 74-420 μm particles, and demineralized Matrix was then bioassayed for its ability to induce endochondral bone on day 11 following subcutaneous implantation over the thorax of Long-Evans rats. The resulting plaques of tissue were subjected to histological analysis, determination of alkaline phosphatase activity, and calcium content. Bone matrix derived from diabetic animals proved to be a significantly better inducer of endochondral bone than did control matrix.",
keywords = "Diabetic bone matrix, Endochondral bone formation, Osteoinductive potential, Streptozotocin-induced diabetes",
author = "Richard Landesman and Reddi, {A Hari}",
year = "1985",
month = "11",
doi = "10.1007/BF02554921",
language = "English (US)",
volume = "37",
pages = "630--634",
journal = "Calcified Tissue International",
issn = "0171-967X",
publisher = "Springer New York",
number = "6",

}

TY - JOUR

T1 - Induction of endochondral bone by demineralized bone matrix from diabetic rats

AU - Landesman, Richard

AU - Reddi, A Hari

PY - 1985/11

Y1 - 1985/11

N2 - In this investigation we examined the osteoinductive potential of demineralized bone matrix derived from chronically diabetic (streptozotocin-induced) rats. Long-Evans rats (28-31 days) were made diabetic with a single injection of streptozotocin (65 mg/kg) and provided food and water ad lib for 2 months. Diaphyseal shafts of femurs and tibias removed from the diabetic rats and their sibling controls were dehydrated, pulverized, sieved to 74-420 μm particles, and demineralized Matrix was then bioassayed for its ability to induce endochondral bone on day 11 following subcutaneous implantation over the thorax of Long-Evans rats. The resulting plaques of tissue were subjected to histological analysis, determination of alkaline phosphatase activity, and calcium content. Bone matrix derived from diabetic animals proved to be a significantly better inducer of endochondral bone than did control matrix.

AB - In this investigation we examined the osteoinductive potential of demineralized bone matrix derived from chronically diabetic (streptozotocin-induced) rats. Long-Evans rats (28-31 days) were made diabetic with a single injection of streptozotocin (65 mg/kg) and provided food and water ad lib for 2 months. Diaphyseal shafts of femurs and tibias removed from the diabetic rats and their sibling controls were dehydrated, pulverized, sieved to 74-420 μm particles, and demineralized Matrix was then bioassayed for its ability to induce endochondral bone on day 11 following subcutaneous implantation over the thorax of Long-Evans rats. The resulting plaques of tissue were subjected to histological analysis, determination of alkaline phosphatase activity, and calcium content. Bone matrix derived from diabetic animals proved to be a significantly better inducer of endochondral bone than did control matrix.

KW - Diabetic bone matrix

KW - Endochondral bone formation

KW - Osteoinductive potential

KW - Streptozotocin-induced diabetes

UR - http://www.scopus.com/inward/record.url?scp=0022386675&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022386675&partnerID=8YFLogxK

U2 - 10.1007/BF02554921

DO - 10.1007/BF02554921

M3 - Article

VL - 37

SP - 630

EP - 634

JO - Calcified Tissue International

JF - Calcified Tissue International

SN - 0171-967X

IS - 6

ER -