Induction of endochondral bone by demineralized bone matrix from diabetic rats

Richard Landesman, A Hari Reddi

Research output: Contribution to journalArticlepeer-review

9 Scopus citations


In this investigation we examined the osteoinductive potential of demineralized bone matrix derived from chronically diabetic (streptozotocin-induced) rats. Long-Evans rats (28-31 days) were made diabetic with a single injection of streptozotocin (65 mg/kg) and provided food and water ad lib for 2 months. Diaphyseal shafts of femurs and tibias removed from the diabetic rats and their sibling controls were dehydrated, pulverized, sieved to 74-420 μm particles, and demineralized Matrix was then bioassayed for its ability to induce endochondral bone on day 11 following subcutaneous implantation over the thorax of Long-Evans rats. The resulting plaques of tissue were subjected to histological analysis, determination of alkaline phosphatase activity, and calcium content. Bone matrix derived from diabetic animals proved to be a significantly better inducer of endochondral bone than did control matrix.

Original languageEnglish (US)
Pages (from-to)630-634
Number of pages5
JournalCalcified Tissue International
Issue number6
StatePublished - Nov 1985
Externally publishedYes


  • Diabetic bone matrix
  • Endochondral bone formation
  • Osteoinductive potential
  • Streptozotocin-induced diabetes

ASJC Scopus subject areas

  • Endocrinology
  • Orthopedics and Sports Medicine
  • Endocrinology, Diabetes and Metabolism


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