Induction of chondrogenesis and superficial zone protein accumulation in synovial side population cells by BMP-7 and TGF-β1

Shintaro Yamane, A Hari Reddi

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Synovial cells are known to contain a sub-population of cells with multipotent differentiating capacity including chondrocytes. However, the stem/progenitor cells in synovial cells have not been well characterized. Stem/progenitor cells can exclude Hoechst 33342 dye, and the cell fraction with this property is called "side population (SP)." SP cells are present in many adult tissues. The aim of this investigation was to identify, isolate, and characterize SP cells from bovine synovium. Hoechst dye efflux and fluorescence activated cell sorting showed that synovial cells contained 0.60% SP cells. In the presence of verapamil, an inhibitor of ABC transporters critical for the dye efflux property, the SP cell fraction was not observed, indicating the critical role of ABC transporters. Isoforms of ABC transporters (ABCG2 and ABCB1 mRNA) were highly expressed in SP cells derived from the synovial cells by real-time RT-PCR analysis. Bone morphogenetic protein-7 (BMP-7) induced type II collagen mRNA expression characteristic of chondrogenesis in articular cartilage with both SP and non-SP cells. In addition, expression of SZP mRNA, a marker of the surface layer of articular cartilage, was significantly up-regulated by BMP-7, and the protein accumulation of SZP was stimulated by both BMP- 7 and TGF-β1. Thus, synovial cells contain ABC transporter-dependent SP cells. These findings demonstrate that side population cells of synovium differentiate toward an articular chondrocyte phenotype of the surface layer and have direct implications for tissue engineering and regeneration of articular cartilage.

Original languageEnglish (US)
Pages (from-to)485-492
Number of pages8
JournalJournal of Orthopaedic Research
Volume26
Issue number4
DOIs
StatePublished - Apr 2008

Fingerprint

Side-Population Cells
Bone Morphogenetic Protein 7
Chondrogenesis
ATP-Binding Cassette Transporters
Articular Cartilage
Proteins
Stem Cells
Coloring Agents
Synovial Membrane
Chondrocytes
Messenger RNA
Population
Collagen Type II
Tissue Engineering
Verapamil
Real-Time Polymerase Chain Reaction
Regeneration
Flow Cytometry
Protein Isoforms
Joints

Keywords

  • ABC transporters
  • Aggrecan
  • Collagen type 2
  • Lubricin
  • Sox9

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine
  • Medicine(all)

Cite this

Induction of chondrogenesis and superficial zone protein accumulation in synovial side population cells by BMP-7 and TGF-β1. / Yamane, Shintaro; Reddi, A Hari.

In: Journal of Orthopaedic Research, Vol. 26, No. 4, 04.2008, p. 485-492.

Research output: Contribution to journalArticle

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